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Literature summary extracted from

  • Vyas, R.; Kumar, V.; Panjikar, S.; Karthikeyan, S.; Kishan, K.V.; Tewari, R.; Weiss, M.S.
    Purification, crystallization and preliminary X-ray diffraction analysis of aspartate semialdehyde dehydrogenase (Rv3708c) from Mycobacterium tuberculosis (2008), Acta Crystallogr. Sect. F, 64, 167-170.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.11 expression of His-tagged enzyme in Escherichia coli strain M15 Mycobacterium tuberculosis
1.2.1.11 heterologous expression in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.11 by using the hanging-drop vapour-diffusion method, crystallization in 2 different crystal forms, diffraction data analysis suggests the presence of up to four monomers in the asymmetric unit of the orthorhombic crystal form and of one or 2 monomers in the cubic crystal form Mycobacterium tuberculosis
1.2.1.11 purified recombinant His-tagged enzyme, 9 mg/ml protein in 10 mM potassium phosphate buffer, pH 8.0, and 10 mM DTT, sitting drop vapour diffusion method, mixing of 500 nl of protein and of reservoir solution, the latter containing 1.6 M ammonium sulfate and 100 mM citric acid, pH 5.0, 2 days at 18°C, two different crystal forms, X-ray diffraction structure determination and analysis at 2.18-2.75 A resolution Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.11 37000
-
 2 * 37000, about, recombinant His-tagged enzyme, SDS-PAGE Mycobacterium tuberculosis
1.2.1.11 38070
-
 the expressed and purified monomer protein construct includes the 17-amino-acid sequence RGSHHHHHHGSACELGT between the N-terminal Met and the second amino acid Gly of the native Asd sequence, the total length of this protein is 362 amino acids Mycobacterium tuberculosis
1.2.1.11 75000
-
 recombinant His-tagged enzyme, gel filtration Mycobacterium tuberculosis
1.2.1.11 75000
-
 homodimer in solution, determined by gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.11 aspartate-4-semialdehyde + phosphate + NADP+ Mycobacterium tuberculosis second enzyme in the lysine/homoserine biosynthetic pathways L-4-aspartyl phosphate + NADPH + H+
-
 r
1.2.1.11 aspartate-4-semialdehyde + phosphate + NADP+ Mycobacterium tuberculosis H37Rv second enzyme in the lysine/homoserine biosynthetic pathways L-4-aspartyl phosphate + NADPH + H+
-
 r
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+ Mycobacterium tuberculosis the enzyme takes part in the lysine/homoserine-biosynthetic pathway L-4-aspartyl phosphate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.11 Mycobacterium tuberculosis
-
 gene Rv3708c
-
1.2.1.11 Mycobacterium tuberculosis P9WNX5
-
-
1.2.1.11 Mycobacterium tuberculosis H37Rv P9WNX5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.11 by using affinity chromatography and gel filtration Mycobacterium tuberculosis
1.2.1.11 recombinant His-tagged enzyme from Escherichia coli strain M15 by nickel affinity chromatography and gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.11 aspartate-4-semialdehyde + phosphate + NADP+ second enzyme in the lysine/homoserine biosynthetic pathways Mycobacterium tuberculosis L-4-aspartyl phosphate + NADPH + H+
-
 r
1.2.1.11 aspartate-4-semialdehyde + phosphate + NADP+ second enzyme in the lysine/homoserine biosynthetic pathways Mycobacterium tuberculosis H37Rv L-4-aspartyl phosphate + NADPH + H+
-
 r
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Mycobacterium tuberculosis L-4-aspartyl phosphate + NADPH + H+
-
 ?
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+ the enzyme takes part in the lysine/homoserine-biosynthetic pathway Mycobacterium tuberculosis L-4-aspartyl phosphate + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.11 dimer 2 * 37000, about, recombinant His-tagged enzyme, SDS-PAGE Mycobacterium tuberculosis
1.2.1.11 homodimer recombinant enzyme Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.2.1.11 ASADH
-
 Mycobacterium tuberculosis
1.2.1.11 Asd
-
 Mycobacterium tuberculosis
1.2.1.11 aspartate semialdehyde dehydrogenase
-
 Mycobacterium tuberculosis
1.2.1.11 aspartate-beta-semialdehyde dehydrogenase
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.11 NADP+
-
 Mycobacterium tuberculosis