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Literature summary extracted from

  • Bajaj, M.; Moriyama, H.
    Purification, crystallization and preliminary crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Arabidopsis thaliana (2007), Acta Crystallogr. Sect. F, 63, 409-411.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.23 enzyme expression Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.23 purified recombinant enzyme, hanging drop vapour diffusion method, 25°C, 0.001 ml of protein solution containing 10 mg/ml protein in 50 mM Tris-HCl, pH 7.4, is mixed with 0.001 ml of reservoir solution containing 2 M ammonium sulfate and 0.1 M Tris-HCl, pH 8.5, X-ray diffraction structure determination and analysis at 2.2-2.3 A resolution, molecular replacement Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.23 dUTP + H2O Arabidopsis thaliana the enzyme is responsible for maintaining a low dUTP level in order to avoid the incorporation of uracil into DNA, and for providing dUMP as a substrate for deoxythymidine triphosphate biosynthesis dUMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Arabidopsis thaliana Q9STG6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.23 recombinant enzyme from to homogeneity Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dUTP + H2O
-
 Arabidopsis thaliana dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O the enzyme is responsible for maintaining a low dUTP level in order to avoid the incorporation of uracil into DNA, and for providing dUMP as a substrate for deoxythymidine triphosphate biosynthesis Arabidopsis thaliana dUMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.23 deoxyuridine triphosphate nucleotidohydrolase
-
 Arabidopsis thaliana
3.6.1.23 dUTPase
-
 Arabidopsis thaliana