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Literature summary extracted from

  • Sato, Y.; Natsume, R.; Tsuda, M.; Damborsky, J.; Nagata, Y.; Senda, T.
    Crystallization and preliminary crystallographic analysis of a haloalkane dehalogenase, DbjA, from Bradyrhizobium japonicum USDA110 (2007), Acta Crystallogr. Sect. F, 63, 294-296.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.8.1.5 environmental protection haloalkane dehalogenases are key enzymes for the degradation of halogenated aliphatic pollutants Bradyrhizobium japonicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.5 the plasmid pYBJA2 is used for expression of the protein in Escherichia coli BL21 cells, the DbjA construct codes for residues 1-310 of DbjA with its C-terminus fused to a His6-tag Bradyrhizobium japonicum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.8.1.5 the crystal diffracts to 1.75 A resolution Bradyrhizobium japonicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.5 1-haloalkane + H2O Bradyrhizobium japonicum
-
 a primary alcohol + halide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.5 Bradyrhizobium japonicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.5 using a Ni Chelating Sepharose Fast Flow column Bradyrhizobium japonicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.5 1-haloalkane + H2O
-
 Bradyrhizobium japonicum a primary alcohol + halide
-
 ?
3.8.1.5 additional information DjA has a high catalytic activity for beta-methylated haloalkanes Bradyrhizobium japonicum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.8.1.5 DbjA
-
 Bradyrhizobium japonicum
3.8.1.5 haloalkane dehalogenase
-
 Bradyrhizobium japonicum