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Literature summary extracted from

  • Martinez-Rodriguez, S.; Gonzalez-Ramirez, L.A.; Clemente-Jimenez, J.M.; Rodriguez-Vico, F.; Las Heras-Vazquez, F.J.; Gavira, J.A.; Garcia-Ruiz, J.M.
    Crystallization and preliminary crystallographic studies of the recombinant dihydropyrimidinase from Sinorhizobium meliloti CECT4114 (2006), Acta Crystallogr. Sect. F, 62, 1223-1226.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.2 expressed in Escherichia coli BL21 cells Sinorhizobium meliloti

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.2.2 hanging drop vapour diffusion method with 4.0 M sodium formate, 0.1 M sodium acetate pH 4.6 as precipitant in the presence of agarose at 0.1% (w/v) Sinorhizobium meliloti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.2 200000
-
 gel filtration Sinorhizobium meliloti

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.2 Sinorhizobium meliloti Q0PQZ5
-
-
3.5.2.2 Sinorhizobium meliloti CECT4114 Q0PQZ5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.2 Talon metalaffinity resin column chromatography and Superdex 200 gel filtration Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.2 5,6-dihydrothymine + H2O
-
 Sinorhizobium meliloti ?
-
 ?
3.5.2.2 5,6-dihydrothymine + H2O
-
 Sinorhizobium meliloti CECT4114 ?
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Sinorhizobium meliloti 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Sinorhizobium meliloti CECT4114 3-ureidopropionate
-
 ?
3.5.2.2 DL-5-isopropylhydantoin + H2O
-
 Sinorhizobium meliloti N-carbamoyl-D-valine
-
 ?
3.5.2.2 DL-5-isopropylhydantoin + H2O
-
 Sinorhizobium meliloti CECT4114 N-carbamoyl-D-valine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.2 tetramer x-ray crystallography Sinorhizobium meliloti

Synonyms

EC Number Synonyms Comment Organism
3.5.2.2 SmelDhp possesses both hydantoinase and dihydropyrimidinase activities Sinorhizobium meliloti