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Literature summary extracted from
- Molecular cloning and functional expression of triterpene synthases from pea (Pisum sativum) new alpha-amyrin-producing enzyme is a multifunctional triterpene synthase (2000), Eur. J. Biochem., 267, 3453-3460.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.39 | expression in Saccharomyces cerevisiae | Pisum sativum |
| 5.4.99.40 | expression of PSM in ERG7 deficient yeast mutant GIL77 | Pisum sativum |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.99.39 | additional information | functional expression in Saccharomyces cerevisiae mutant lacking lanosterol synthase activity results in production of beta-amyrin | Pisum sativum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.40 | (3S)-2,3-epoxy-2,3-dihydrosqualene | Pisum sativum | PSM produces both alpha- and beta-amyrin in about 3 : 2 ratio together with at least six other minor triterpenes | alpha-amyrin | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.39 | Pisum sativum | - |
- |
- |
| 5.4.99.40 | Pisum sativum | Q9LRH7 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.4.99.39 | seed | immature seed | Pisum sativum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.39 | 2,3-oxidosqualene | - |
Pisum sativum | beta-amyrin | sole product | ? | |
| 5.4.99.40 | (3S)-2,3-epoxy-2,3-dihydrosqualene | PSM produces both alpha- and beta-amyrin in about 3 : 2 ratio together with at least six other minor triterpenes | Pisum sativum | alpha-amyrin | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.39 | PSY | - |
Pisum sativum |
| 5.4.99.40 | 2,3-oxidosqualene alpha-amyrin cyclase | - |
Pisum sativum |
| 5.4.99.40 | mixed amyrin synthase | a multifunctional enzyme which produces both alpha- and beta-amyrin (see EC 5.4.99.39, beta-amyrin synthase) | Pisum sativum |
| 5.4.99.40 | OSCPSM | - |
Pisum sativum |
| 5.4.99.40 | PSM | - |
Pisum sativum |
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