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Literature summary extracted from

  • Zeller, M.E.; Csanadi, A.; Miczak, A.; Rose, T.; Bizebard, T.; Kaberdin, V.R.
    Quaternary structure and biochemical properties of mycobacterial RNase E/G (2007), Biochem. J., 403, 207-215.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.24 a 1.8 kb DNA fragment, encoding the C-terminal portion of the MycRne polypeptide, residues 332-953, is cloned into p6HisF-11d for expression in Escherichia coli BL21-CodonPlusDE3-RIL cells Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.6.1.24 Mg2+
-
 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.6.1.24 71100
-
 apparent molecular mass of the monomer, determined by sedimentation equilibrium analysis Mycobacterium tuberculosis
4.6.1.24 325000
-
 tetramer, determined by gel-filtration Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.24 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.24 usung a HisTrap HP column Mycobacterium tuberculosis

Storage Stability

EC Number Storage Stability Organism
4.6.1.24 -80°C, HEPES buffer, pH 7.5, 0.1 mM EDTA, 1 mM DTT, 50 mM NaCl Mycobacterium tuberculosis

Subunits

EC Number Subunits Comment Organism
4.6.1.24 dimer
-
 Mycobacterium tuberculosis
4.6.1.24 tetramer
-
 Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
4.6.1.24 MycRne
-
 Mycobacterium tuberculosis
4.6.1.24 RNase E/G
-
 Mycobacterium tuberculosis