EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.78 | expression in Escherichia coli | Bacillus subtilis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.78 | Ag+ | 1 mM, 3% residual activity | Bacillus subtilis | |
3.2.1.78 | Hg2+ | 1 mM, 2% residual activity | Bacillus subtilis | |
3.2.1.78 | additional information | not inhibitory: EDTA, 2-mercaptoethanol | Bacillus subtilis | |
3.2.1.78 | sodium dodecylsulfate | 1 mM, 79% residual activity | Bacillus subtilis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.78 | extracellular | - |
Bacillus subtilis | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.78 | 38000 | - |
x * 38013, calculated, x * 38000, SDS-PAGE, mature protein | Bacillus subtilis |
3.2.1.78 | 38013 | - |
x * 38013, calculated, x * 38000, SDS-PAGE, mature protein | Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.78 | Bacillus subtilis | - |
- |
- |
3.2.1.78 | Bacillus subtilis B36 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.78 | from culture supernatant | Bacillus subtilis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.78 | culture fluid | - |
Bacillus subtilis | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.78 | 928 | - |
pH 6.4, 50°C | Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.78 | guar gum + H2O | 21% of the activity with locust bean gum | Bacillus subtilis | ? | - |
? | |
3.2.1.78 | guar gum + H2O | 21% of the activity with locust bean gum | Bacillus subtilis B36 | ? | - |
? | |
3.2.1.78 | locust bean gum + H2O | enzyme is highly specific for substrate | Bacillus subtilis | ? | - |
? | |
3.2.1.78 | locust bean gum + H2O | enzyme is highly specific for substrate | Bacillus subtilis B36 | ? | - |
? | |
3.2.1.78 | additional information | no substrate: carboxymethyl cellulose | Bacillus subtilis | ? | - |
? | |
3.2.1.78 | additional information | no substrate: carboxymethyl cellulose | Bacillus subtilis B36 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.78 | ? | x * 38013, calculated, x * 38000, SDS-PAGE, mature protein | Bacillus subtilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.78 | 50 | - |
- |
Bacillus subtilis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.78 | 60 | - |
60 min, 90% residual activity | Bacillus subtilis |
3.2.1.78 | 70 | - |
30 min, 20% residual activity | Bacillus subtilis |
3.2.1.78 | 80 | - |
20 min, 18% residual activity, 30 min, complete loss of activity | Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.78 | 6.4 | - |
- |
Bacillus subtilis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.78 | 5 | 8 | more than 70% of maximum activity | Bacillus subtilis |
3.2.1.78 | 10 | - |
44% of maximum activity | Bacillus subtilis |