Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hwang, J.; Seo, D.; Kim, J.
    Soluble forms of YlCrh1p and YlCrh2p, cell wall proteins of Yarrowia lipolytica, have beta-1,3-glycosidase activity (2006), Yeast, 23, 803-812.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Yarrowia lipolytica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 ammonium sulfate precipitation and His affinity chromatography using ProBond nickel-chelating resin Yarrowia lipolytica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 403
-
 purified YlCrh2S, using laminarin as substrate, at 30°C Yarrowia lipolytica
3.2.1.39 3349
-
 purified YlCrh1S, using laminarin as substrate, at 30°C Yarrowia lipolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 carboxymethyl-cellulose + H2O carboxymethyl-cellulose is only hydrolyzed by YlCrh1Sp Yarrowia lipolytica ?
-
 ?
3.2.1.39 laminarin + H2O
-
 Yarrowia lipolytica D-glucose + ?
-
 ?
3.2.1.39 lichenan + H2O lichenan is only hydrolyzed by YlCrh1Sp Yarrowia lipolytica ?
-
 ?
3.2.1.39 additional information YlCrh2Sp does not hydrolyze carboxymethyl-cellulose, pustulan, and lichenan Yarrowia lipolytica ?
-
 ?
3.2.1.39 pustulan + H2O pustulan is only hydrolyzed by YlCrh1Sp Yarrowia lipolytica ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 YlCrh1Sp soluble enzyme form lacking the C-terminal consensus sequence for GPI anchoring and showing glycosidase activity on laminarin Yarrowia lipolytica
3.2.1.39 YlCrh2Sp soluble enzyme form lacking the C-terminal consensus sequence for GPI anchoring and showing glycosidase activity on laminarin Yarrowia lipolytica