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Literature summary extracted from

  • Wang, Y.; Fuchs, E.; da Silva, R.; McDaniel, A.; Seibel, J.; Ford, C.
    Improvement of Aspergillus niger glucoamylase thermostability by directed evolution (2006), Starch Staerke, 58, 501-508.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.3 industry glucoamylase is used industrially to catalyze the hydrolysis of alpha-1,4-glycosidic bonds to release beta-D-glucose from the nonreducing ends of starch or oligosaccharides Aspergillus niger

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.3 DNA and amino acid sequence determination and anaylsis of wild-type and mutant enzymes, expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain C468 using vector YEpPM18 Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.3 D20C/A27C/S30P/G137A site-directed mutagenesis, the mutant, designated THS8, is highly thermotolerant with increased stability at 80°C compared to the wild-type enzyme Aspergillus niger
3.2.1.3 H391Y random mutagenesis, the mutant shows increased thermotolerance compared to the wild-type enzyme Aspergillus niger
3.2.1.3 additional information random mutagenesis and mutant screening by plate thermostability assay for increased thermotolerance at 65-80°C, overview Aspergillus niger
3.2.1.3 T290A random mutagenesis, the mutant shows increased thermotolerance compared to the wild-type enzyme Aspergillus niger
3.2.1.3 T62A random mutagenesis, the mutant shows increased thermotolerance compared to the wild-type enzyme Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 0.76
-
 maltose pH 4.5, 35°C, recombinant mutant T62A Aspergillus niger
3.2.1.3 0.78
-
 maltose pH 4.5, 35°C, recombinant mutant T290A Aspergillus niger
3.2.1.3 0.82
-
 maltose pH 4.5, 35°C, recombinant mutant H391Y Aspergillus niger
3.2.1.3 0.83
-
 maltose pH 4.5, 35°C, recombinant wild-type enzyme Aspergillus niger
3.2.1.3 0.88
-
 maltose pH 4.5, 35°C, recombinant mutant S30P/T62A/H391Y Aspergillus niger
3.2.1.3 0.89
-
 maltose pH 4.5, 35°C, recombinant mutant S119P Aspergillus niger
3.2.1.3 1
-
 maltose pH 4.5, 35°C, recombinant mutant D20C/A27C/S30P/G137A Aspergillus niger
3.2.1.3 1.16
-
 maltose pH 4.5, 35°C, recombinant mutant S30P/T290A/H391Y Aspergillus niger
3.2.1.3 1.19
-
 maltose pH 4.5, 35°C, recombinant mutant S30P/H391Y Aspergillus niger
3.2.1.3 1.35
-
 maltose pH 4.5, 35°C, recombinant mutant S30P/T290A Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.3 starch + H2O Aspergillus niger
-
 beta-D-glucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Aspergillus niger
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.3 glycoprotein
-
 Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 13.3
-
 recombinant mutant S30P/T62A/H391Y Aspergillus niger
3.2.1.3 13.6
-
 recombinant mutant T62A Aspergillus niger
3.2.1.3 14
-
 recombinant mutant T290A Aspergillus niger
3.2.1.3 14.2
-
 recombinant wild-type enzyme Aspergillus niger
3.2.1.3 16.8
-
 recombinant mutant S119P Aspergillus niger
3.2.1.3 16.9
-
 recombinant mutant D20C/A27C/S30P/G137A Aspergillus niger
3.2.1.3 17.7
-
 recombinant mutant H391Y Aspergillus niger
3.2.1.3 17.9
-
 recombinant mutant S30P/T290A Aspergillus niger
3.2.1.3 18.1
-
 recombinant mutant S30P/T290A/H391Y Aspergillus niger
3.2.1.3 19.5
-
 recombinant mutant S30P/H391Y Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 maltose + H2O
-
 Aspergillus niger beta-D-glucose + D-glucose
-
 ?
3.2.1.3 starch + H2O
-
 Aspergillus niger beta-D-glucose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 glucoamylase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 50
-
 assay at Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 70 80 irreversible thermoinactivation kinetic analysis of recombinant wild-type and mutant enzymes, overview Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.3 0.03 0.55 maltose pH 4.5, 35°C, recombinant mutant T290A Aspergillus niger
3.2.1.3 0.031 0.51 maltose pH 4.5, 35°C, recombinant wild-type enzyme Aspergillus niger
3.2.1.3 0.04 1.97 maltose pH 4.5, 35°C, recombinant mutant S30P/T62A/H391Y Aspergillus niger
3.2.1.3 0.04 1.97 maltose pH 4.5, 35°C, recombinant mutant T62A Aspergillus niger
3.2.1.3 0.052 2.1 maltose pH 4.5, 35°C, recombinant mutant S30P/H391Y Aspergillus niger
3.2.1.3 0.057 0.65 maltose pH 4.5, 35°C, recombinant mutant D20C/A27C/S30P/G137A Aspergillus niger
3.2.1.3 0.5
-
 maltose pH 4.5, 35°C, recombinant mutant S119P Aspergillus niger
3.2.1.3 7.35
-
 maltose pH 4.5, 35°C, recombinant mutant S30P/T290A Aspergillus niger
3.2.1.3 7.46
-
 maltose pH 4.5, 35°C, recombinant mutant T290A Aspergillus niger
3.2.1.3 7.55
-
 maltose pH 4.5, 35°C, recombinant wild-type enzyme Aspergillus niger
3.2.1.3 8.99
-
 maltose pH 4.5, 35°C, recombinant mutant T62A Aspergillus niger
3.2.1.3 9.3
-
 maltose pH 4.5, 35°C, recombinant mutant S30P/T290A/H391Y Aspergillus niger
3.2.1.3 9.42
-
 maltose pH 4.5, 35°C, recombinant mutant S30P/T62A/H391Y Aspergillus niger
3.2.1.3 10.13
-
 maltose pH 4.5, 35°C, recombinant mutant S30P/H391Y Aspergillus niger
3.2.1.3 10.5
-
 maltose pH 4.5, 35°C, recombinant mutant H391Y Aspergillus niger
3.2.1.3 11.32
-
 maltose pH 4.5, 35°C, recombinant mutant D20C/A27C/S30P/G137A Aspergillus niger
3.2.1.3 13.31
-
 maltose pH 4.5, 35°C, recombinant mutant S119P Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 4.4 4.5 assay at Aspergillus niger