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Literature summary extracted from

  • Swartz, T.E.; Tseng, T.; Frederickson, M.A.; Paris, G.; Comerci, D.J.; Rajashekara, G.; Kim, J.; Mudgett, M.B.; Splitter, G.A.; Ugalde, R.A.; Goldbaum, F.A.; Briggs, W.R.; Bogomolni, R.A.
    Blue-light-activated histidine kinases: Two-component sensors in Bacteria (2007), Science, 317, 1090-1093.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.13.3 additional information light-activated Brucella abortus
2.7.13.3 additional information light-activated Pseudomonas syringae
2.7.13.3 additional information light-activated Erythrobacter litoralis
2.7.13.3 additional information light-activated, 2fold increase in transcription of the LOV-HK gene at a pH similar to that within infected macrophages Brucella melitensis

Application

EC Number Application Comment Organism
2.7.13.3 additional information a LOV domain at the N-terminus, followed by a PAS domain in the intervening sequence, and a histidine kinase at the C-terminus Brucella abortus
2.7.13.3 additional information a LOV domain at the N-terminus, followed by a PAS domain in the intervening sequence, and a histidine kinase at the C-terminus Brucella melitensis
2.7.13.3 additional information LOV domain at the N terminus, followed by a histidine kinase and a putative C-terminal receiver domain Pseudomonas syringae
2.7.13.3 additional information LOV domain followed by a linking sequence and a C-terminal histidine kinase domain Erythrobacter litoralis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.13.3 EL368-LOV-HK and EL346-LOV-HK expressed in Escherichia coli Erythrobacter litoralis
2.7.13.3 LOV-HK expressed in Escherichia coli Pseudomonas syringae
2.7.13.3 LOV-HK expressed in Escherichia coli Brucella melitensis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.13.3 C69A LOV-HK mutant, binds flavin mononucleotide but cannot form the cysteinyl adduct, does not activate the kinase in response to illumination Brucella melitensis
2.7.13.3 C69A mutant in LOV-HK knockout background does not return the replication rate to the wild-type level Brucella abortus
2.7.13.3 additional information insertion-knockout mutant strain by introduction of a kanamycin-resistant cassette into the gene, replication is less than the wild-type strain as early as 4 hours after infection with J774A.1 murine macrophages Brucella abortus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.13.3 additional information in the dark, roughly an order of magnitude fewer bacteria survive, a survival rate no better than that of the LOV-HK knockout mutant Brucella abortus

Organism

EC Number Organism UniProt Comment Textmining
2.7.13.3 Brucella abortus
-
-
-
2.7.13.3 Brucella melitensis
-
-
-
2.7.13.3 Erythrobacter litoralis
-
-
-
2.7.13.3 Pseudomonas syringae
-
-
-
2.7.13.3 Pseudomonas syringae DC3000
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.13.3 full-length protein affinity-purified Pseudomonas syringae
2.7.13.3 full-length protein affinity-purified Brucella melitensis
2.7.13.3 full-length protein affinity-purified Erythrobacter litoralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.13.3 additional information upon illumination in the presence of ATP, the enzyme undergoes autophosphorylation Brucella abortus ?
-
 ?
2.7.13.3 additional information upon illumination in the presence of ATP, the enzyme undergoes autophosphorylation Pseudomonas syringae ?
-
 ?
2.7.13.3 additional information upon illumination in the presence of ATP, the enzyme undergoes autophosphorylation Brucella melitensis ?
-
 ?
2.7.13.3 additional information upon illumination in the presence of ATP, the enzyme undergoes autophosphorylation Erythrobacter litoralis ?
-
 ?
2.7.13.3 additional information upon illumination in the presence of ATP, the enzyme undergoes autophosphorylation Pseudomonas syringae DC3000 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.13.3 histidine kinase
-
 Brucella abortus
2.7.13.3 histidine kinase
-
 Pseudomonas syringae
2.7.13.3 histidine kinase
-
 Brucella melitensis
2.7.13.3 histidine kinase
-
 Erythrobacter litoralis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.13.3 flavin
-
 Pseudomonas syringae
2.7.13.3 flavin
-
 Erythrobacter litoralis
2.7.13.3 flavin flavin-containing histidine kinase functions as a photoreceptor, the flavin chromophore, upon illumination, forms a cysteinyl-flavin adduct, which is the signaling state that activates the histidine kinase Brucella melitensis
2.7.13.3 flavin flavin-containing histidine kinase functions as a photoreceptor, the flavin chromophore, upon illumination, forms a cysteinyl-flavin adduct, which is the signaling state that activates the histidine kinase, regulates Brucella abortus virulence Brucella abortus