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Literature summary extracted from

  • Ochiai, A.; Hashimoto, W.; Murata, K.
    A biosystem for alginate metabolism in Agrobacterium tumefaciens strain C58: molecular identification of Atu3025 as an exotype family PL-15 alginate lyase (2006), Res. Microbiol., 157, 642-649.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.3 overexpressed in Escherichia coli Agrobacterium tumefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.3 D-galacturonic acid
-
 Agrobacterium tumefaciens
4.2.2.3 D-glucuronic acid
-
 Agrobacterium tumefaciens
4.2.2.3 Hg2+ 1 mM, 96% inhibition Agrobacterium tumefaciens
4.2.2.3 Zn2+ 1 mM, complete inhibition Agrobacterium tumefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.3 87871
-
 4 * 87871, calculated from sequence Agrobacterium tumefaciens
4.2.2.3 88000
-
 4 * 88000, SDS-PAGE Agrobacterium tumefaciens
4.2.2.3 350000
-
 gel filtration Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.2.3 alginate Agrobacterium tumefaciens Atu3025 is an exotype alginate lyase potentially involved in the assimilation of low-molecular-weight alginate in strain C58 ?
-
 ?
4.2.2.3 alginate Agrobacterium tumefaciens C58 / ATCC 33970 Atu3025 is an exotype alginate lyase potentially involved in the assimilation of low-molecular-weight alginate in strain C58 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Agrobacterium tumefaciens
-
-
-
4.2.2.3 Agrobacterium tumefaciens C58 / ATCC 33970
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3
-
 Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alginate Atu3025 is an exotype alginate lyase potentially involved in the assimilation of low-molecular-weight alginate in strain C58 Agrobacterium tumefaciens ?
-
 ?
4.2.2.3 alginate Atu3025 is an exotype family PL-15 alginate lyase Agrobacterium tumefaciens ?
-
 ?
4.2.2.3 alginate Atu3025 is an exotype alginate lyase potentially involved in the assimilation of low-molecular-weight alginate in strain C58 Agrobacterium tumefaciens C58 / ATCC 33970 ?
-
 ?
4.2.2.3 alginate Atu3025 is an exotype family PL-15 alginate lyase Agrobacterium tumefaciens C58 / ATCC 33970 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 tetramer 4 * 88000, SDS-PAGE Agrobacterium tumefaciens
4.2.2.3 tetramer 4 * 87871, calculated from sequence Agrobacterium tumefaciens

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 Atu3025
-
 Agrobacterium tumefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 30
-
-
 Agrobacterium tumefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 7.3
-
-
 Agrobacterium tumefaciens