Literature summary extracted from
Guo, J.P.; Ma, Y.
High-level expression, purification and characterization of recombinant Aspergillus oryzae alkaline protease in Pichia pastoris (2008), Protein Expr. Purif., 58, 301-308.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.4.21.63 |
high-level expression of plasmids pPIC9K-n/Alp and pPIC9K-alpha/Alp in Pichia pastoris GS115 with native signal peptide or alpha-factor secretion signal peptide |
Aspergillus oryzae |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.4.21.63 |
Ag+ |
strong inhibitor of recombinant and native enzyme |
Aspergillus oryzae |
|
3.4.21.63 |
benzamidine |
slightly inhibits recombinant and native enzyme |
Aspergillus oryzae |
|
3.4.21.63 |
Cu2+ |
inhibitor of recombinant and native enzyme |
Aspergillus oryzae |
|
3.4.21.63 |
EDTA |
slightly inhibits recombinant and native enzyme |
Aspergillus oryzae |
|
3.4.21.63 |
Hg2+ |
strong inhibitor of recombinant and native enzyme |
Aspergillus oryzae |
|
3.4.21.63 |
Mn2+ |
inhibitor of recombinant and native enzyme |
Aspergillus oryzae |
|
3.4.21.63 |
N-tosyl-L-lysine chloromethyl ketone |
inhibitor of recombinant and native enzyme |
Aspergillus oryzae |
|
3.4.21.63 |
phenylmethylsulfonyl fluoride |
strong inhibitor of recombinant and native enzyme |
Aspergillus oryzae |
|
3.4.21.63 |
Zn2+ |
inhibitor of recombinant and native enzyme |
Aspergillus oryzae |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.4.21.63 |
Ca2+ |
stimulates recombinant and native enzyme |
Aspergillus oryzae |
|
3.4.21.63 |
Mg2+ |
stimulates recombinant and native enzyme |
Aspergillus oryzae |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.4.21.63 |
34000 |
- |
x * 34000, recombinant enzyme, SDS-PAGE |
Aspergillus oryzae |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.21.63 |
Aspergillus oryzae |
P12547 |
strain Y2942 |
- |
3.4.21.63 |
Aspergillus oryzae RIB 40 |
P12547 |
strain Y2942 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.4.21.63 |
by ammonium sulfate precipitation, ion exchange chromatography and gel filtration |
Aspergillus oryzae |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.4.21.63 |
40.94 |
- |
culture supernatant |
Aspergillus oryzae |
3.4.21.63 |
92.73 |
- |
purified recombinant enzyme |
Aspergillus oryzae |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.4.21.63 |
casein + H2O |
- |
Aspergillus oryzae |
hydrolyzed casein |
- |
? |
|
3.4.21.63 |
casein + H2O |
- |
Aspergillus oryzae RIB 40 |
hydrolyzed casein |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.4.21.63 |
? |
x * 34000, recombinant enzyme, SDS-PAGE |
Aspergillus oryzae |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.21.63 |
Alkaline protease |
- |
Aspergillus oryzae |
3.4.21.63 |
ALP |
- |
Aspergillus oryzae |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.4.21.63 |
40 |
- |
recombinant and native enzyme |
Aspergillus oryzae |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
3.4.21.63 |
40 |
60 |
recombinant and native enzyme |
Aspergillus oryzae |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.4.21.63 |
additional information |
- |
the native enzyme exhibits a better thermostability than the recombinant enzyme |
Aspergillus oryzae |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.4.21.63 |
9 |
- |
recombinant and native enzyme |
Aspergillus oryzae |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
3.4.21.63 |
7 |
10 |
recombinant and native enzyme |
Aspergillus oryzae |