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Literature summary extracted from
- High-level expression, purification and characterization of recombinant Aspergillus oryzae alkaline protease in Pichia pastoris (2008), Protein Expr. Purif., 58, 301-308.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.21.63 | high-level expression of plasmids pPIC9K-n/Alp and pPIC9K-alpha/Alp in Pichia pastoris GS115 with native signal peptide or alpha-factor secretion signal peptide | Aspergillus oryzae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.63 | Ag+ | strong inhibitor of recombinant and native enzyme | Aspergillus oryzae |  |
| 3.4.21.63 | benzamidine | slightly inhibits recombinant and native enzyme | Aspergillus oryzae | |
| 3.4.21.63 | Cu2+ | inhibitor of recombinant and native enzyme | Aspergillus oryzae | |
| 3.4.21.63 | EDTA | slightly inhibits recombinant and native enzyme | Aspergillus oryzae | |
| 3.4.21.63 | Hg2+ | strong inhibitor of recombinant and native enzyme | Aspergillus oryzae | |
| 3.4.21.63 | Mn2+ | inhibitor of recombinant and native enzyme | Aspergillus oryzae | |
| 3.4.21.63 | N-tosyl-L-lysine chloromethyl ketone | inhibitor of recombinant and native enzyme | Aspergillus oryzae | |
| 3.4.21.63 | phenylmethylsulfonyl fluoride | strong inhibitor of recombinant and native enzyme | Aspergillus oryzae | |
| 3.4.21.63 | Zn2+ | inhibitor of recombinant and native enzyme | Aspergillus oryzae | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.63 | Ca2+ | stimulates recombinant and native enzyme | Aspergillus oryzae | |
| 3.4.21.63 | Mg2+ | stimulates recombinant and native enzyme | Aspergillus oryzae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.63 | 34000 | - |
x * 34000, recombinant enzyme, SDS-PAGE | Aspergillus oryzae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.63 | Aspergillus oryzae | P12547 | strain Y2942 | - |
| 3.4.21.63 | Aspergillus oryzae RIB 40 | P12547 | strain Y2942 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.63 | by ammonium sulfate precipitation, ion exchange chromatography and gel filtration | Aspergillus oryzae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.63 | 40.94 | - |
culture supernatant | Aspergillus oryzae |
| 3.4.21.63 | 92.73 | - |
purified recombinant enzyme | Aspergillus oryzae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.63 | casein + H2O | - |
Aspergillus oryzae | hydrolyzed casein | - |
? | |
| 3.4.21.63 | casein + H2O | - |
Aspergillus oryzae RIB 40 | hydrolyzed casein | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.63 | ? | x * 34000, recombinant enzyme, SDS-PAGE | Aspergillus oryzae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.63 | Alkaline protease | - |
Aspergillus oryzae |
| 3.4.21.63 | ALP | - |
Aspergillus oryzae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.63 | 40 | - |
recombinant and native enzyme | Aspergillus oryzae |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.63 | 40 | 60 | recombinant and native enzyme | Aspergillus oryzae |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.63 | additional information | - |
the native enzyme exhibits a better thermostability than the recombinant enzyme | Aspergillus oryzae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.63 | 9 | - |
recombinant and native enzyme | Aspergillus oryzae |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.63 | 7 | 10 | recombinant and native enzyme | Aspergillus oryzae |
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