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Literature summary extracted from

  • Yu, M.; Lange, S.; Richter, S.; Tan, T.; Schmid, R.D.
    High-level expression of extracellular lipase Lip2 from Yarrowia lipolytica in Pichia pastoris and its purification and characterization (2007), Protein Expr. Purif., 53, 255-263.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 gene lip2, phylogenetic tree, overexpression in Pichia pastoris strain X-33 using the Saccharomyces cerevisiae secretion signal peptide, alpha-factor, under the control of the methanol inducible promoter of the alcohol oxidase 1 gene, AOX1, the recombinant enzyme is secreted to the culture medium Yarrowia lipolytica

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Yarrowia lipolytica
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.3 glycoprotein 12% sugar content in native and recombinant enzymes, deglycosylation with endoglycosidase H Yarrowia lipolytica

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 recombinant enzyme 3.34fold from Pichia pastoris strain X-33 cell culture medium by ultrafiltration and anion exchange chromatography Yarrowia lipolytica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 19880
-
purified extracellular enzyme Yarrowia lipolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 methyl myristate + H2O best fatty acid methyl ester substrate Yarrowia lipolytica methanol + myristate
-
?
3.1.1.3 additional information the lipase showed high activity toward long-chain fatty acid methyl esters with C12–C16, overview Yarrowia lipolytica ?
-
?
3.1.1.3 olive oil + H2O best substrate Yarrowia lipolytica ?
-
?
3.1.1.3 tributyrin + H2O
-
Yarrowia lipolytica dibutyrin + butyrate
-
?
3.1.1.3 tricaprylin + H2O
-
Yarrowia lipolytica dicaprylin + caprylate
-
?
3.1.1.3 trilaurin + H2O low activity Yarrowia lipolytica dilaurin + laurate
-
?
3.1.1.3 tripalmitin + H2O low activity Yarrowia lipolytica dipalmitin + palmitate
-
?
3.1.1.3 tristearin + H2O low activity Yarrowia lipolytica distearin + stearate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 extracellular lipase
-
Yarrowia lipolytica
3.1.1.3 LIP2
-
Yarrowia lipolytica
3.1.1.3 YlLip2
-
Yarrowia lipolytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 40
-
-
Yarrowia lipolytica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 25 55 activity range, temperature profile, overview Yarrowia lipolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
-
Yarrowia lipolytica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 3.5 9 activity range, pH profile, overview Yarrowia lipolytica