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Literature summary extracted from

  • Boer, H.; Simolin, H.; Cottaz, S.; Soederlund, H.; Koivula, A.
    Heterologous expression and site-directed mutagenesis studies of two Trichoderma harzianum chitinases, Chit33 and Chit42, in Escherichia coli (2007), Protein Expr. Purif., 51, 216-226.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 expression in Escherichia coli. Cytoplasmic overexpression results in inclusion body formation. Active enzyme can be recovered after refolding. Periplasmic proves to be better suited for mutagenesis purposes Trichoderma harzianum

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.14 D170N 0.6% of wild-type activity Trichoderma harzianum
3.2.1.14 E172Q 0.3% of wild-type activity Trichoderma harzianum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 allosamidin
-
 Trichoderma harzianum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 0.9
-
 4-nitrophenyl 2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-beta-D-glucopyranoside pH 6.5, wild-type enzyme from Trichoderma harzianum Trichoderma harzianum
3.2.1.14 1.1
-
 4-nitrophenyl 2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-beta-D-glucopyranoside pH 6.5, wilde-type recombinant enzyme expressed in Escherichia coli Trichoderma harzianum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Trichoderma harzianum P48827
-
-
3.2.1.14 Trichoderma harzianum Q12713
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.14 additional information Chit33 does not contain any putative N-glycosylation site Trichoderma harzianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O essential role of the two carboxylic acid residues E172 and D170 in the catalytic mechanism of Chit42 Trichoderma harzianum ?
-
 ?
3.2.1.14 additional information essential role of E172, D170 and E145 in the catalytic mechanism of Chit33 Trichoderma harzianum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 Chit33
-
 Trichoderma harzianum
3.2.1.14 CHIT42
-
 Trichoderma harzianum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 38
-
 enzyme form Trichoderma harzianum Trichoderma harzianum
3.2.1.14 47
-
 enzyme recombinantly expressed in Escherichia coli and refolded Trichoderma harzianum
3.2.1.14 50
-
 enzyme recombinantly expressed in Escherichia coli and refolded Trichoderma harzianum
3.2.1.14 55
-
 enzyme form Trichoderma harzianum Trichoderma harzianum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 23 50 23°C: about 50% of maximal activity, 50°C: about 30% of maximal activity, enzyme form Trichoderma harzianum Trichoderma harzianum
3.2.1.14 23 50 23°C: about 60% of maximal activity, 50°C: about 30% of maximal activity, enzyme recombinantly expressed in Escherichia coli and refolded Trichoderma harzianum
3.2.1.14 40 55 40°C: about 80% of maximal activity, 55°C: about 90% of maximal activity, 65°C: no activity, enzyme form Trichoderma harzianum Trichoderma harzianum
3.2.1.14 40 60 40°C: about 70% of maximal activity, 60°C: about 80% of maximal activity, 70°C, no activity, enzyme recombinantly expressed in Escherichia coli and refolded Trichoderma harzianum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.14 0.267
-
 4-nitrophenyl 2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-beta-D-glucopyranoside pH 6.5, wild-type enzyme from Trichoderma harzianum Trichoderma harzianum
3.2.1.14 0.35
-
 4-nitrophenyl 2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-beta-D-glucopyranoside pH 6.5, wilde-type recombinant enzyme expressed in Escherichia coli Trichoderma harzianum