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Literature summary extracted from

  • Song, H.T.; Jiang, Z.B.; Ma, L.X.
    Expression and purification of two lipases from Yarrowia lipolytica AS 2.1216 (2006), Protein Expr. Purif., 47, 393-397.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 gene lip7, DNA and amino acid sequence determination and anaylsis, expression of His6-tagged LipY7p in Pichia pastoris strain KM71, expression pattern, overview Yarrowia lipolytica
3.1.1.3 gene lip8, DNA and amino acid sequence determination and anaylsis, expression of His6-tagged LipY8p in Pichia pastoris strain KM71, expression pattern, overview Yarrowia lipolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 41000
-
 x * 41000, recombinant LipY7p, SDS-PAGE Yarrowia lipolytica
3.1.1.3 41000
-
 x * 41000, recombinant LipY8p, SDS-PAGE Yarrowia lipolytica

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 DMSO at 50%, 3 h, room temperature, purified recombinant LipY7p, over 60% remaining activity Yarrowia lipolytica
3.1.1.3 DMSO at 50%, 3 h, room temperature, purified recombinant LipY8p, over 60% remaining activity Yarrowia lipolytica

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Yarrowia lipolytica Q872L3 LIPY8
-
3.1.1.3 Yarrowia lipolytica Q872L4 LipY7p
-
3.1.1.3 Yarrowia lipolytica AS 2.1216 Q872L3 LIPY8
-
3.1.1.3 Yarrowia lipolytica AS 2.1216 Q872L4 LipY7p
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.3 glycoprotein the recombinant LipY7p expressed in Pichia pastoris is slightly glycosylated Yarrowia lipolytica
3.1.1.3 glycoprotein the recombinant LipY8p expressed in Pichia pastoris is slightly glycosylated Yarrowia lipolytica

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 recombinant His6-tagged LipY7p from Pichia pastoris strain KM71 by nickel affinity chromatography Yarrowia lipolytica
3.1.1.3 recombinant His6-tagged LipY8p from Pichia pastoris strain KM71 by nickel affinity chromatography Yarrowia lipolytica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 57.8
-
 purified recombinant LipY8p Yarrowia lipolytica
3.1.1.3 62.6
-
 purified recombinant LipY7p Yarrowia lipolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Yarrowia lipolytica 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Yarrowia lipolytica AS 2.1216 4-nitrophenol + laurate
-
 ?
3.1.1.3 additional information substrate specificity of LipY8p, overview Yarrowia lipolytica ?
-
 ?
3.1.1.3 additional information substrate specificity of LipY8p, overview Yarrowia lipolytica AS 2.1216 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 41000, recombinant LipY7p, SDS-PAGE Yarrowia lipolytica
3.1.1.3 ? x * 41000, recombinant LipY8p, SDS-PAGE Yarrowia lipolytica

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Yarrowia lipolytica
3.1.1.3 LipY7p
-
 Yarrowia lipolytica
3.1.1.3 LipY8p
-
 Yarrowia lipolytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 40
-
-
 Yarrowia lipolytica
3.1.1.3 45
-
-
 Yarrowia lipolytica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 65
-
 3 h, purified recombinant LipY7p, pH 7.0, 85% remaining activity Yarrowia lipolytica
3.1.1.3 65
-
 3 h, purified recombinant LipY8p, pH 7.0, 85% remaining activity Yarrowia lipolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
 assay at Yarrowia lipolytica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 5 10 3 h, room temperature, recombinant LipY7p, 75% remaining activity Yarrowia lipolytica
3.1.1.3 5 10 3 h, room temperature, recombinant LipY8p, 75% remaining activity Yarrowia lipolytica