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Literature summary extracted from

  • Yu, M.; Qin, S.; Tan, T.
    Purification and characterization of the extracellular lipase Lip2 from Yarrowia lipolytica (2007), Process Biochem., 42, 384-391.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 additional information the LIP2 is not affected by EDTA, SDS, Tween 80, Span 65, Span 85, Triton X-100, DTT, and 2-mercaptoethanol Yarrowia lipolytica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 gene lip2, DNA and amino acid sequence determination and analysis Yarrowia lipolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Cu2+
-
Yarrowia lipolytica
3.1.1.3 Fe2+ slight inhibition Yarrowia lipolytica
3.1.1.3 K+ slight inhibition Yarrowia lipolytica
3.1.1.3 additional information the LIP2 is not affected by Mg2+, Mn2+, EDTA, SDS, Tween 80, Span 65, Span 85, Triton X-100, DTT, and 2-mercaptoethanol Yarrowia lipolytica
3.1.1.3 Ni2+
-
Yarrowia lipolytica
3.1.1.3 Zn2+
-
Yarrowia lipolytica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular
-
Yarrowia lipolytica
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Ca2+ activates 1.23fold at 5 mM Yarrowia lipolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 38000
-
x * 38000, LIP2, SDS-PAGE Yarrowia lipolytica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.3 olive oil + H2O Yarrowia lipolytica
-
?
-
?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 Acetone 30°C, 30 min, about 90% remaining activity at 10%, inactivation at 20% Yarrowia lipolytica
3.1.1.3 DMSO 30°C, 30 min, about 90% remaining activity at 10%, 95% activity at 20% Yarrowia lipolytica
3.1.1.3 Ethanol 30°C, 30 min, about 90% remaining activity at 10%, inactivation at 20% Yarrowia lipolytica
3.1.1.3 isopropanol 30°C, 30 min, about 90% remaining activity at 10%, inactivation at 20% Yarrowia lipolytica
3.1.1.3 Methanol 30°C, 30 min, about 90% remaining activity at 10%, 60% activity at 20% Yarrowia lipolytica

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Yarrowia lipolytica Q9P8F7
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.3 glycoprotein LIP2 is a glycosylated protein which contains about 12% sugar, the deglycosylated lipase retains 88% specific activity compared to the untreated lipase Yarrowia lipolytica

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 native extracellular enzyme 26.5fold by solvent precipitation, anion exchange and hydrophobic interaction chromatography Yarrowia lipolytica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 21300
-
purified enzyme, substrate olive oil Yarrowia lipolytica
3.1.1.3 22900
-
purified enzyme, substrate safflower oil Yarrowia lipolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 flax seed oil + H2O
-
Yarrowia lipolytica ?
-
?
3.1.1.3 methyl myristate + H2O best fatty acid methyl ester substrate Yarrowia lipolytica methanol + myristate
-
?
3.1.1.3 additional information the lipase showed a preference for long chain fatty acid methyl esters of C12-C16, substrate specificity, overview Yarrowia lipolytica ?
-
?
3.1.1.3 olive oil + H2O
-
Yarrowia lipolytica ?
-
?
3.1.1.3 safflower oil + H2O
-
Yarrowia lipolytica ?
-
?
3.1.1.3 soybean oil + H2O
-
Yarrowia lipolytica ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 38000, LIP2, SDS-PAGE Yarrowia lipolytica

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 extracellular lipase
-
Yarrowia lipolytica
3.1.1.3 LIP2
-
Yarrowia lipolytica
3.1.1.3 YlLip2
-
Yarrowia lipolytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 40
-
-
Yarrowia lipolytica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 25 45 sharp drop of activity above 45°C, inactive at 60°C Yarrowia lipolytica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 30 45 4 h, 30°C and 35°C, the purified lipase retains 95% and 83% activity, respectively, 32% and 5% remaining activity after 4 h at 40°C and 45°C, respectively Yarrowia lipolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
-
Yarrowia lipolytica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 5.5 9 activity range, the activity drops off rapidly at pH above 8.0 with only about 20% of maximal activity remaining at pH 9.0 Yarrowia lipolytica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 4 10 purified enzyme, 3 h, 25°C, stable Yarrowia lipolytica