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Literature summary extracted from

  • Gote, M.M.; Khan, M.I.; Gokhale, D.V.; Bastawde, K.B.; Khire, J.M.
    Purification, characterization and substrate specificity of thermostable alpha-galactosidase from Bacillus stearothermophilus (NCIM-5146) (2006), Process Biochem., 41, 1311-1317.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 Ag+ potent inhibitor Geobacillus stearothermophilus
3.2.1.22 Cu2+ potent inhibitor Geobacillus stearothermophilus
3.2.1.22 D-galactose
-
Geobacillus stearothermophilus
3.2.1.22 Hg2+ potent inhibitor Geobacillus stearothermophilus
3.2.1.22 lactose
-
Geobacillus stearothermophilus
3.2.1.22 melibiose
-
Geobacillus stearothermophilus
3.2.1.22 additional information beta-mercaptoethanol (1.0 mM) and urea (1.0 M) do not inhibit the enzyme activity Geobacillus stearothermophilus
3.2.1.22 PCMB potent inhibitor Geobacillus stearothermophilus
3.2.1.22 stachyose
-
Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.22 additional information Ba2+, Ca2+, Co2+, Fe3+, K+, Mg2+, Mn2+, Na+, Ni2+, Zn2+ (1 mM) and EDTA (10 mM) have no influence on enzyme activity Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 79900
-
SDS-PAGE Geobacillus stearothermophilus
3.2.1.22 82950
-
2 * 82950, gel filtration Geobacillus stearothermophilus
3.2.1.22 165900
-
gel filtration Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Geobacillus stearothermophilus
-
-
-
3.2.1.22 Geobacillus stearothermophilus NCIM-5146
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22 phenyl Sepharose CL-4B column chromatography Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 1.03
-
culture filtrate Geobacillus stearothermophilus
3.2.1.22 400
-
after 388fold purification Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 melibiose + H2O
-
Geobacillus stearothermophilus D-galactose + D-glucose
-
?
3.2.1.22 melibiose + H2O
-
Geobacillus stearothermophilus NCIM-5146 D-galactose + D-glucose
-
?
3.2.1.22 additional information no detectable activity towards methyl-alpha-D-galactopyranoside, locust bean gum, guar gum, and alpha-D-Gal-(1,4)-D-Gal Geobacillus stearothermophilus ?
-
?
3.2.1.22 additional information no detectable activity towards methyl-alpha-D-galactopyranoside, locust bean gum, guar gum, and alpha-D-Gal-(1,4)-D-Gal Geobacillus stearothermophilus NCIM-5146 ?
-
?
3.2.1.22 o-nitrophenyl alpha-D-galactopyranoside + H2O
-
Geobacillus stearothermophilus o-nitrophenol + alpha-D-galactose
-
?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O
-
Geobacillus stearothermophilus p-nitrophenol + alpha-D-galactose
-
?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O
-
Geobacillus stearothermophilus NCIM-5146 p-nitrophenol + alpha-D-galactose
-
?
3.2.1.22 raffinose + H2O
-
Geobacillus stearothermophilus sucrose + D-galactose
-
?
3.2.1.22 raffinose + H2O
-
Geobacillus stearothermophilus NCIM-5146 sucrose + D-galactose
-
?
3.2.1.22 stachyose + 2 H2O
-
Geobacillus stearothermophilus 2 alpha-D-galactose + sucrose
-
?
3.2.1.22 stachyose + 2 H2O
-
Geobacillus stearothermophilus NCIM-5146 2 alpha-D-galactose + sucrose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 dimer 2 * 82950, gel filtration Geobacillus stearothermophilus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.22 Alpha-D-galactoside galactohydrolase
-
Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 65
-
-
Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 70
-
half-life of inactivation at 70°C is 30 min Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 6.5 7
-
Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.22 3 9
-
Geobacillus stearothermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.22 14.5
-
lactose
-
Geobacillus stearothermophilus
3.2.1.22 16.25
-
D-galactose
-
Geobacillus stearothermophilus
3.2.1.22 16.25
-
melibiose
-
Geobacillus stearothermophilus
3.2.1.22 33
-
stachyose
-
Geobacillus stearothermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.22 Geobacillus stearothermophilus isoelectric focusing
-
4.9