EC Number | Application | Comment | Organism |
---|---|---|---|
4.1.99.3 | biotechnology | a new class of photolyases with specificity for cyclobutane pyrimidine dimers in ssDNA is defined. Members of these branch are found in bacteria, plants, and animals, and are designated Cry-DASH, because of the lack of significant photorepair activity on dsDNA | Arabidopsis thaliana |
4.1.99.3 | biotechnology | a new class of photolyases with specificity for cyclobutane pyrimidine dimers in ssDNA is defined. Members of these branch are found in bacteria, plants, and animals, and are designated Cry-DASH, because of the lack of significant photorepair activity on dsDNA | Xenopus laevis |
4.1.99.3 | biotechnology | a new class of photolyases with specificity for cyclobutane pyrimidine dimers in ssDNA is defined. Members of these branch are found in bacteria, plants, and animals, and are designated Cry-DASH, because of the lack of significant photorepair activity on dsDNA | Vibrio cholerae serotype O1 |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.2.14 | His6-tagged KDPG aldolase is cloned in Escherichia coli BL21 | Thermotoga maritima |
4.1.99.3 | AtCry1 belonging to the cryptochrome/photolyase class of enzymes called Cry-DASH proteins is shown to possess no photorepair activity under any condition | Arabidopsis thaliana |
4.1.99.3 | AtCry3 belonging to the cryptochrome/photolyase class of enzymes called Cry-DASH proteins is shown to be photolyases with high degree of specificity for cyclobutane pyrimidine dimers in ssDNA | Arabidopsis thaliana |
4.1.99.3 | BsUvrC belonging to the cryptochrome/photolyase class of enzymes called Cry-DASH proteins is shown to possess no photorepair activity under any condition | Bacillus subtilis |
4.1.99.3 | VcCry1 belonging to the cryptochrome/photolyase class of enzymes called Cry-DASH proteins is shown to be photolyases with high degree of specificity for cyclobutane pyrimidine dimers in ssDNA | Vibrio cholerae serotype O1 |
4.1.99.3 | XlCry-DASH belonging to the cryptochrome/photolyase class of enzymes called Cry-DASH proteins is shown to be photolyases with high degree of specificity for cyclobutane pyrimidine dimers in ssDNA | Xenopus laevis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.14 | 0.1 | - |
2-keto-3-deoxy-6-phosphogluconate | - |
Thermotoga maritima |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.2.14 | Thermotoga maritima | - |
- |
- |
4.1.99.3 | Arabidopsis thaliana | - |
- |
- |
4.1.99.3 | Bacillus subtilis | - |
- |
- |
4.1.99.3 | Vibrio cholerae serotype O1 | - |
- |
- |
4.1.99.3 | Xenopus laevis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.2.14 | purified by using Ni2+ affinity column | Thermotoga maritima |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.99.3 | additional information | - |
AtCry1 shows no photorepair activity under any condition | Arabidopsis thaliana |
4.1.99.3 | additional information | - |
BsUvrC shows no photorepair activity under any condition | Bacillus subtilis |
4.1.99.3 | additional information | - |
it is shown by in vitro and in vivo by photoreactivation assays and electrophoretic mobility shift assays that AtCry3, as a member of the Cry-DASH enzymes, is specific for cyclobutane pyrimidine dimers in ssDNA | Arabidopsis thaliana |
4.1.99.3 | additional information | - |
it is shown by in vitro and in vivo by photoreactivation assays and electrophoretic mobility shift assays that VcCry1, as a member of the Cry-DASH enzymes,is specific for cyclobutane pyrimidine dimers in ssDNA | Vibrio cholerae serotype O1 |
4.1.99.3 | additional information | - |
it is shown by in vitro and in vivo by photoreactivation assays and electrophoretic mobility shift assays that XlCry-DASH, as a member of the Cry-DASH enzymes, is specific for cyclobutane pyrimidine dimers in ssDNA | Xenopus laevis |
4.1.99.3 | additional information | - |
it is shown that VcCry1 repairs photodimers in the deoxyribonucleotide homopolymers poly dU (uracil cyclobutane dimer) and poly dT much more efficiently than the dimers in the ribopolynucleotide homopolymer | Vibrio cholerae serotype O1 |
EC Number | Storage Stability | Organism |
---|---|---|
4.1.2.14 | protein can be stored at 4°C for up to 6 months without a significant loss in activity. | Thermotoga maritima |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.14 | 2-dehydro-3-deoxy-6-phospho-D-gluconate | - |
Thermotoga maritima | pyruvate + D-glyceraldehyde 3-phosphate | - |
? | |
4.1.2.14 | 2-pyridinecarboxyaldehyde + pyruvate | typical isolated yield of product is 80-90% after reverse-phase C18 purification. Reaction is also doable with substrates other than 2-pyridinecarboxyaldehyde. Enantiomeric excess greater than 99.7% | Thermotoga maritima | (S)-4-hydroxy-2-keto-4-(2'-pyridyl)butyrate | - |
? | |
4.1.99.3 | cyclobutadipyrimidine in DNA | AtCry3 repairs the dimer but only in ssDNA | Arabidopsis thaliana | 2 pyrimidine residues in DNA | - |
? | |
4.1.99.3 | cyclobutadipyrimidine in DNA | VcCry1 repairs the dimer but only in ssDNA | Vibrio cholerae serotype O1 | 2 pyrimidine residues in DNA | - |
? | |
4.1.99.3 | cyclobutadipyrimidine in DNA | XlCry-DASH repairs the dimer but only in ssDNA | Xenopus laevis | 2 pyrimidine residues in DNA | - |
? | |
4.1.99.3 | cyclobutadipyrimidine in RNA | - |
Arabidopsis thaliana | 2 pyrimidine residues in RNA | - |
? | |
4.1.99.3 | cyclobutadipyrimidine in RNA | - |
Xenopus laevis | 2 pyrimidine residues in RNA | - |
? | |
4.1.99.3 | cyclobutadipyrimidine in RNA | - |
Vibrio cholerae serotype O1 | 2 pyrimidine residues in RNA | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.2.14 | 2-keto-3-deoxy-6-phosphogluconate aldolase | - |
Thermotoga maritima |
4.1.2.14 | KDPG aldolase | - |
Thermotoga maritima |
4.1.99.3 | AtCry1 | - |
Arabidopsis thaliana |
4.1.99.3 | AtCry3 | - |
Arabidopsis thaliana |
4.1.99.3 | BsUvrC | - |
Bacillus subtilis |
4.1.99.3 | Cry-DASH | - |
Bacillus subtilis |
4.1.99.3 | Cry-DASH | - |
Arabidopsis thaliana |
4.1.99.3 | Cry-DASH | - |
Xenopus laevis |
4.1.99.3 | Cry-DASH | - |
Vibrio cholerae serotype O1 |
4.1.99.3 | VcCry1 | - |
Vibrio cholerae serotype O1 |
4.1.99.3 | XlCry-DASH | - |
Xenopus laevis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.14 | 80 | - |
2-keto-3-deoxy-6-phosphogluconate | - |
Thermotoga maritima |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.99.3 | 7.5 | - |
assay at | Arabidopsis thaliana |
4.1.99.3 | 7.5 | - |
assay at | Xenopus laevis |
4.1.99.3 | 7.5 | - |
assay at | Vibrio cholerae serotype O1 |