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Literature summary extracted from

  • Selby, C.P.; Sancar, A.
    A cryptochrome/photolyase class of enzymes with single-stranded DNA-specific photolyase activity (2006), Proc. Natl. Acad. Sci. USA, 103, 17696-17700.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.1.99.3 biotechnology a new class of photolyases with specificity for cyclobutane pyrimidine dimers in ssDNA is defined. Members of these branch are found in bacteria, plants, and animals, and are designated Cry-DASH, because of the lack of significant photorepair activity on dsDNA Arabidopsis thaliana
4.1.99.3 biotechnology a new class of photolyases with specificity for cyclobutane pyrimidine dimers in ssDNA is defined. Members of these branch are found in bacteria, plants, and animals, and are designated Cry-DASH, because of the lack of significant photorepair activity on dsDNA Xenopus laevis
4.1.99.3 biotechnology a new class of photolyases with specificity for cyclobutane pyrimidine dimers in ssDNA is defined. Members of these branch are found in bacteria, plants, and animals, and are designated Cry-DASH, because of the lack of significant photorepair activity on dsDNA Vibrio cholerae serotype O1

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.14 His6-tagged KDPG aldolase is cloned in Escherichia coli BL21 Thermotoga maritima
4.1.99.3 AtCry1 belonging to the cryptochrome/photolyase class of enzymes called Cry-DASH proteins is shown to possess no photorepair activity under any condition Arabidopsis thaliana
4.1.99.3 AtCry3 belonging to the cryptochrome/photolyase class of enzymes called Cry-DASH proteins is shown to be photolyases with high degree of specificity for cyclobutane pyrimidine dimers in ssDNA Arabidopsis thaliana
4.1.99.3 BsUvrC belonging to the cryptochrome/photolyase class of enzymes called Cry-DASH proteins is shown to possess no photorepair activity under any condition Bacillus subtilis
4.1.99.3 VcCry1 belonging to the cryptochrome/photolyase class of enzymes called Cry-DASH proteins is shown to be photolyases with high degree of specificity for cyclobutane pyrimidine dimers in ssDNA Vibrio cholerae serotype O1
4.1.99.3 XlCry-DASH belonging to the cryptochrome/photolyase class of enzymes called Cry-DASH proteins is shown to be photolyases with high degree of specificity for cyclobutane pyrimidine dimers in ssDNA Xenopus laevis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.14 0.1
-
2-keto-3-deoxy-6-phosphogluconate
-
Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.14 Thermotoga maritima
-
-
-
4.1.99.3 Arabidopsis thaliana
-
-
-
4.1.99.3 Bacillus subtilis
-
-
-
4.1.99.3 Vibrio cholerae serotype O1
-
-
-
4.1.99.3 Xenopus laevis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.14 purified by using Ni2+ affinity column Thermotoga maritima

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.99.3 additional information
-
AtCry1 shows no photorepair activity under any condition Arabidopsis thaliana
4.1.99.3 additional information
-
BsUvrC shows no photorepair activity under any condition Bacillus subtilis
4.1.99.3 additional information
-
it is shown by in vitro and in vivo by photoreactivation assays and electrophoretic mobility shift assays that AtCry3, as a member of the Cry-DASH enzymes, is specific for cyclobutane pyrimidine dimers in ssDNA Arabidopsis thaliana
4.1.99.3 additional information
-
it is shown by in vitro and in vivo by photoreactivation assays and electrophoretic mobility shift assays that VcCry1, as a member of the Cry-DASH enzymes,is specific for cyclobutane pyrimidine dimers in ssDNA Vibrio cholerae serotype O1
4.1.99.3 additional information
-
it is shown by in vitro and in vivo by photoreactivation assays and electrophoretic mobility shift assays that XlCry-DASH, as a member of the Cry-DASH enzymes, is specific for cyclobutane pyrimidine dimers in ssDNA Xenopus laevis
4.1.99.3 additional information
-
it is shown that VcCry1 repairs photodimers in the deoxyribonucleotide homopolymers poly dU (uracil cyclobutane dimer) and poly dT much more efficiently than the dimers in the ribopolynucleotide homopolymer Vibrio cholerae serotype O1

Storage Stability

EC Number Storage Stability Organism
4.1.2.14 protein can be stored at 4°C for up to 6 months without a significant loss in activity. Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.14 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
Thermotoga maritima pyruvate + D-glyceraldehyde 3-phosphate
-
?
4.1.2.14 2-pyridinecarboxyaldehyde + pyruvate typical isolated yield of product is 80-90% after reverse-phase C18 purification. Reaction is also doable with substrates other than 2-pyridinecarboxyaldehyde. Enantiomeric excess greater than 99.7% Thermotoga maritima (S)-4-hydroxy-2-keto-4-(2'-pyridyl)butyrate
-
?
4.1.99.3 cyclobutadipyrimidine in DNA AtCry3 repairs the dimer but only in ssDNA Arabidopsis thaliana 2 pyrimidine residues in DNA
-
?
4.1.99.3 cyclobutadipyrimidine in DNA VcCry1 repairs the dimer but only in ssDNA Vibrio cholerae serotype O1 2 pyrimidine residues in DNA
-
?
4.1.99.3 cyclobutadipyrimidine in DNA XlCry-DASH repairs the dimer but only in ssDNA Xenopus laevis 2 pyrimidine residues in DNA
-
?
4.1.99.3 cyclobutadipyrimidine in RNA
-
Arabidopsis thaliana 2 pyrimidine residues in RNA
-
?
4.1.99.3 cyclobutadipyrimidine in RNA
-
Xenopus laevis 2 pyrimidine residues in RNA
-
?
4.1.99.3 cyclobutadipyrimidine in RNA
-
Vibrio cholerae serotype O1 2 pyrimidine residues in RNA
-
?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.14 2-keto-3-deoxy-6-phosphogluconate aldolase
-
Thermotoga maritima
4.1.2.14 KDPG aldolase
-
Thermotoga maritima
4.1.99.3 AtCry1
-
Arabidopsis thaliana
4.1.99.3 AtCry3
-
Arabidopsis thaliana
4.1.99.3 BsUvrC
-
Bacillus subtilis
4.1.99.3 Cry-DASH
-
Bacillus subtilis
4.1.99.3 Cry-DASH
-
Arabidopsis thaliana
4.1.99.3 Cry-DASH
-
Xenopus laevis
4.1.99.3 Cry-DASH
-
Vibrio cholerae serotype O1
4.1.99.3 VcCry1
-
Vibrio cholerae serotype O1
4.1.99.3 XlCry-DASH
-
Xenopus laevis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.14 80
-
2-keto-3-deoxy-6-phosphogluconate
-
Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.99.3 7.5
-
assay at Arabidopsis thaliana
4.1.99.3 7.5
-
assay at Xenopus laevis
4.1.99.3 7.5
-
assay at Vibrio cholerae serotype O1