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Literature summary extracted from

  • Ferrari, S.; Galletti, R.; Pontiggia, D.; Manfredini, C.; Lionetti, V.; Bellincampi, D.; Cervone, F.; De Lorenzo, G.
    Transgenic expression of a fungal endo-polygalacturonase increases plant resistance to pathogens and reduces auxin sensitivity (2008), Plant Physiol., 146, 669-681.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.15 agriculture PG plants exhibit enhanced resistance to the necrotrophic fungal pathogen Botrytis cinerea and to the virulent bacterial pathogen Pseudomonas syringae and have constitutively activated defense responses by releasing oligogalacturonides Aspergillus niger

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.15 generation of transgenic Arabidopsis thaliana Ws and Nicotiana tabacum Petit Havana-SR1 plants expressing PG with the point mutation N178D. Arabidopsis line PG201 expressing PGII with the point mutation D201N Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.15 D201N inactive, point mutation in the catalytic site that causes complete loss of enzymatic activity Aspergillus niger
3.2.1.15 N178D has an activity approximately 20fold lower than the native Aspergillus niger PGII Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.15 auxin either in tobacco PG or wild-type plants treated with oligogalacturonides, resistance to fungal infection is suppressed by exogenous auxin, whereas sensitivity to auxin of PG plants is reduced in different bioassays. Altered auxin sensitivity in PG plants may be due to an increased accumulation of oligogalacturonides and subsequent antagonism of auxin action Aspergillus niger
3.2.1.15 additional information the resistant phenotype is not exhibited by transgenic tobacco plants expressing both PG and its inhibitor PvPGIP2 or by Arabidopsis plants expressing a mutagenized and inactive AnPGII (PG201) Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Aspergillus niger
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.15 leaf
-
Aspergillus niger
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 homogalacturonan + H2O
-
Aspergillus niger oligogalacturonide
-
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Synonyms

EC Number Synonyms Comment Organism
3.2.1.15 PGII
-
Aspergillus niger