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Literature summary extracted from

  • Loivamaeki, M.; Gilmer, F.; Fischbach, R.J.; Soergel, C.; Bachl, A.; Walter, A.; Schnitzler, J.P.
    Arabidopsis, a model to study biological functions of isoprene emission? (2007), Plant Physiol., 144, 1066-1078.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.27 into the pQE50 vector for expression in Escherichia coli, into the binary vector pBinAR for transformation of Agrobacterium tumefaciens and generating transgenic Arabidopsis thaliana plants Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate Arabidopsis thaliana
-
 isoprene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.27 Arabidopsis thaliana
-
 transgenic, transformend with Grey poplar ISPS
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.27 leaf of transgenic Arabidopsis thaliana Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.27 0.000006
-
 PcISPS activity detected from 2-week-old plant material, Arabidopsis thaliana line 5 Arabidopsis thaliana
4.2.3.27 0.000012
-
 PcISPS activity detected from 2-week-old plant material, Arabidopsis thaliana line 10 Arabidopsis thaliana
4.2.3.27 0.000042
-
 PcISPS activity detected from 2-week-old plant material, Arabidopsis thaliana line 3 Arabidopsis thaliana
4.2.3.27 0.000072
-
 PcISPS activity detected from 2-week-old plant material, Arabidopsis thaliana line 9 Arabidopsis thaliana
4.2.3.27 0.00014
-
 PcISPS activity detected from 2-week-old plant material, Arabidopsis thaliana line 8 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate
-
 Arabidopsis thaliana isoprene + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.27 isoprene synthase
-
 Arabidopsis thaliana
4.2.3.27 ISPS
-
 Arabidopsis thaliana
4.2.3.27 PcISPS
-
 Arabidopsis thaliana