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Literature summary extracted from

  • Loivamaeki, M.; Louis, S.; Cinege, G.; Zimmer, I.; Fischbach, R.J.; Schnitzler, J.P.
    Circadian rhythms of isoprene biosynthesis in grey poplar leaves (2007), Plant Physiol., 143, 540-551.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.27 light
-
Populus tremula x Populus alba

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate Populus tremula x Populus alba
-
isoprene + diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.27 Populus tremula x Populus alba Q7XAS7 Grey poplar
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.27 0.000024
-
continuous light condition, time curve over 72h, experiment 2 Populus tremula x Populus alba
4.2.3.27 0.000027
-
continuous dark condition, time curve over 72h Populus tremula x Populus alba
4.2.3.27 0.000036
-
continuous light condition, time curve over 72h, experiment 1 Populus tremula x Populus alba

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate
-
Populus tremula x Populus alba isoprene + diphosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.27 isoprene synthase
-
Populus tremula x Populus alba
4.2.3.27 ISPS
-
Populus tremula x Populus alba
4.2.3.27 PcISPS
-
Populus tremula x Populus alba

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.27 additional information
-
additional information turnover values ranging from 3.7 to 5.1 mmol isoprene mol-1 ISPS under darkness, long-day conditions, i.e. 16 h light, 8 h dark, and continuous light conditions Populus tremula x Populus alba

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.27 8
-
activity assay Populus tremula x Populus alba