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Literature summary extracted from

  • Chen, H.; Gonzales-Vigil, E.; Wilkerson, C.G.; Howe, G.A.
    Stability of plant defense proteins in the gut of insect herbivores (2007), Plant Physiol., 143, 1954-1967.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.19 a modified SlTD2 cDNA is cloned into the pET30a+ vector producing a truncated form of SlTD2 lacking the 51 amino acids corresponding to the N-terminal chloroplast-targeting sequence Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.19 2.3
-
L-threonine processed TD2 Solanum lycopersicum
4.3.1.19 3
-
L-serine processed TD2 Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.19 40000
-
molecular mass of processed TD2 determined by SDS-PAGE Solanum lycopersicum
4.3.1.19 55000
-
determined by Western blot analysis, mature TD2 Solanum lycopersicum
4.3.1.19 66180
-
predicted molecular mass of SlTD1 Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.19 L-threonine Solanum lycopersicum
-
2-oxobutanoate + NH3
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.19 Solanum lycopersicum P25306
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.19 using a Sephadex G-25, a DEAE-cellulose DE52, a HiLoad26/60 Superdex 200 and a HiPrep 16/10 column Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.1.19 leaf
-
Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.19 L-serine
-
Solanum lycopersicum pyruvate + NH3
-
?
4.3.1.19 L-threonine
-
Solanum lycopersicum 2-oxobutanoate + NH3
-
?

Synonyms

EC Number Synonyms Comment Organism
4.3.1.19 pTD2 processed form of TD2, the C-terminal regulatory domain is removed Solanum lycopersicum
4.3.1.19 SlTD1 isoform, involved in isoleucine biosynthesis in plants Solanum lycopersicum
4.3.1.19 SlTD2 isoform, for plant defense the protein is involved in threonine degradation in leaf eating insects Solanum lycopersicum
4.3.1.19 TD
-
Solanum lycopersicum
4.3.1.19 TD1
-
Solanum lycopersicum
4.3.1.19 TD2
-
Solanum lycopersicum
4.3.1.19 Threonine deaminase
-
Solanum lycopersicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.19 58
-
-
Solanum lycopersicum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.3.1.19 additional information
-
active over a wide range of temperatures, optimal enzyme activity is observed at 58°C Solanum lycopersicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.3.1.19 additional information
-
the enzyme is highly active in an alkaline pH range, little or no activity is observed at pH values below 6.0 Solanum lycopersicum