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Literature summary extracted from

  • Grigoreva, N.V.; Kondrateva, T.F.; Krasilnikova, E.N.; Karavaiko, G.I.
    Mechanism of cyanide and thiocyanate decomposition by an association of Pseudomonas putida and Pseudomonas stutzeri strains (2006), Microbiology, 75, 266-273.
No PubMed abstract available

Application

EC Number Application Comment Organism
4.2.1.104 biotechnology analysis of strain characteristics for biotechnological application, detoxification of cyanide- or thiocyanate-containing soils and industrial effluents Pseudomonas putida
4.2.1.104 biotechnology analysis of strain characteristics for biotechnological application, detoxification of cyanide- or thiocyanate-containing soils and industrial effluents Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.104 NCO- + HCO3- + 2 H+ Pseudomonas stutzeri utilization of CN– and SCN– as nitrogen sources, cyanase activity present if grown with SCN- and CN- NH3 + 2 CO2 NH4 and CO2 are terminal nitrogen and carbon products of CN– and SCN– decomposition ?
4.2.1.104 NCO- + HCO3- + 2 H+ Pseudomonas stutzeri 18 utilization of CN– and SCN– as nitrogen sources, cyanase activity present if grown with SCN- and CN- NH3 + 2 CO2 NH4 and CO2 are terminal nitrogen and carbon products of CN– and SCN– decomposition ?
4.2.1.104 NCO- + HCO3- + H+ Pseudomonas putida utilization of CN- and SCN- as nitrogen sources, cyanase activity present if grown with SCN- and CN- NH3 + CO2 NH4 and CO2 are terminal nitrogen and carbon products of CN- and SCN- decomposition ?
4.2.1.104 NCO- + HCO3- + H+ Pseudomonas putida 21 utilization of CN- and SCN- as nitrogen sources, cyanase activity present if grown with SCN- and CN- NH3 + CO2 NH4 and CO2 are terminal nitrogen and carbon products of CN- and SCN- decomposition ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.104 Pseudomonas putida
-
-
-
4.2.1.104 Pseudomonas putida
-
 isolated from environments under anthropogenic contaminations, selected by resistance to cyanide
-
4.2.1.104 Pseudomonas putida 21
-
-
-
4.2.1.104 Pseudomonas putida 21
-
 isolated from environments under anthropogenic contaminations, selected by resistance to cyanide
-
4.2.1.104 Pseudomonas stutzeri
-
-
-
4.2.1.104 Pseudomonas stutzeri
-
 associated strains, isolated from environments under anthropogenic contaminations, selected by resistance to cyanide
-
4.2.1.104 Pseudomonas stutzeri 18
-
-
-
4.2.1.104 Pseudomonas stutzeri 18
-
 associated strains, isolated from environments under anthropogenic contaminations, selected by resistance to cyanide
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.104 additional information cyanase activity is present in the cells grown with SCN– but absent in cells grown with NH4+ Pseudomonas putida
-
4.2.1.104 additional information cyanase activity is present in the cells grown with SCN– but absent in cells grown with NH4+ Pseudomonas stutzeri
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.104 additional information
-
 strain 21 of Pseudomonas putida is a more active CN– decomposer than strain 18 of Pseudomonas stutzeri Pseudomonas putida
4.2.1.104 additional information
-
 strain 21 of Pseudomonas putida is a more active CN– decomposer than strain 18 of Pseudomonas stutzeri Pseudomonas stutzeri
4.2.1.104 0.75
-
-
 Pseudomonas putida
4.2.1.104 0.75
-
 activity determined every 15 to 30 min in the presence of 2 mM KCNO and 2 mM HCO3- by formation of NH4+, absent activity if bacteria are grown with NH4 indicates inducible synthesis of cyanase Pseudomonas putida
4.2.1.104 1.26
-
-
 Pseudomonas stutzeri
4.2.1.104 1.26
-
 pH optimum 8.1, activity determined every 15 to 30 min in the presence of 2 mM KCNO and 2 mM HCO3- by formation of NH4+, pH values of 7.0, 8.0 and 9.1, absent activity if bacteria are grown with NH4+ indicates inducible synthesis of cyanase Pseudomonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.104 NCO- + HCO3- + 2 H+ utilization of CN– and SCN– as nitrogen sources, cyanase activity present if grown with SCN- and CN- Pseudomonas stutzeri NH3 + 2 CO2 NH4 and CO2 are terminal nitrogen and carbon products of CN– and SCN– decomposition ?
4.2.1.104 NCO- + HCO3- + 2 H+ utilization of CN– and SCN– as nitrogen sources, cyanase activity present if grown with SCN- and CN- Pseudomonas stutzeri 18 NH3 + 2 CO2 NH4 and CO2 are terminal nitrogen and carbon products of CN– and SCN– decomposition ?
4.2.1.104 NCO- + HCO3- + H+ utilization of CN- and SCN- as nitrogen sources, cyanase activity present if grown with SCN- and CN- Pseudomonas putida NH3 + CO2 NH4 and CO2 are terminal nitrogen and carbon products of CN- and SCN- decomposition ?
4.2.1.104 NCO- + HCO3- + H+ studies on cyanide and thiocyanate decomposition in Pseudomonas Pseudomonas putida NH3 + CO2 after supply of CN- and SCN-, production of NH4+ observed at pH 8.8 in phosphate buffer, due to high rate of their utilization as N-source NH3 and CNO– are absent from the culture liquids ?
4.2.1.104 NCO- + HCO3- + H+ studies on cyanide and thiocyanate decomposition in Pseudomonas Pseudomonas stutzeri NH3 + CO2 after supply of CN– and SCN–, production of NH4+ observed at pH 8.8 in phosphate buffer ?
4.2.1.104 NCO- + HCO3- + H+ studies on cyanide and thiocyanate decomposition in Pseudomonas Pseudomonas stutzeri 18 NH3 + CO2 after supply of CN– and SCN–, production of NH4+ observed at pH 8.8 in phosphate buffer ?
4.2.1.104 NCO- + HCO3- + H+ utilization of CN- and SCN- as nitrogen sources, cyanase activity present if grown with SCN- and CN- Pseudomonas putida 21 NH3 + CO2 NH4 and CO2 are terminal nitrogen and carbon products of CN- and SCN- decomposition ?
4.2.1.104 NCO- + HCO3- + H+ studies on cyanide and thiocyanate decomposition in Pseudomonas Pseudomonas putida 21 NH3 + CO2 after supply of CN- and SCN-, production of NH4+ observed at pH 8.8 in phosphate buffer, due to high rate of their utilization as N-source NH3 and CNO– are absent from the culture liquids ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.104 cyanase
-
 Pseudomonas putida
4.2.1.104 cyanase
-
 Pseudomonas stutzeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.104 8.1
-
-
 Pseudomonas putida
4.2.1.104 8.1
-
-
 Pseudomonas stutzeri
4.2.1.104 8.1
-
 phosphate buffer Pseudomonas putida
4.2.1.104 8.1
-
 phosphate buffer Pseudomonas stutzeri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.104 additional information
-
 assay at pH values of 7.0, 8.0 and 9.1, no activity at pH 7.0, activity at pH 9.1 is 15.8% to 16% of the maximum Pseudomonas putida
4.2.1.104 additional information
-
 no activity at pH 7.0, activity at pH 9.1 is 15.8% to 16% of the maximum Pseudomonas stutzeri
4.2.1.104 9.1
-
 activity at pH 9.1 ia 16% of the activity at maximum Pseudomonas putida
4.2.1.104 9.1
-
 activity at pH 9.1 ia 16% of the activity at maximum Pseudomonas stutzeri