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Literature summary extracted from

  • Madhusudhan, K.T.; Sokatch, J.R.
    Purification of branched-chain keto acid dehydrogenase regulator from Pseudomonas putida (2000), Methods Enzymol., 324, 329-335.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.4.4 expressed in Escherichia coli strain DH5alpha Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.4.4 19850
-
 4 * 19850, gel filtration Pseudomonas putida
1.2.4.4 20000
-
 4 * 20000, SDS-PAGE Pseudomonas putida
1.2.4.4 79400
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.2.4.4 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.4.4 DEAE-Sepharose CL-6B column chromatography and heparin-Sepharose CL-6B column chromatography Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.2.4.4 -70°C, 10 mM Tris-HC1, pH 8.0, containing 0.01 mM EDTA, 0.2 M NaCI, 0.005% (v/v) 2-mercaptoethanol, and 50% (v/v) glycerol, 2 years, remains active Pseudomonas putida

Subunits

EC Number Subunits Comment Organism
1.2.4.4 homotetramer 4 * 20000, SDS-PAGE Pseudomonas putida
1.2.4.4 homotetramer 4 * 19850, gel filtration Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.2.4.4 Bkd
-
 Pseudomonas putida
1.2.4.4 branched-chain keto acid dehydrogenase contains three components: E1 (alpha2/beta2, dehydrogenase-decarboxylase), E2 (transacylase), and E3 (lipoamide dehydrogenase) Pseudomonas putida