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Literature summary extracted from

  • Zhou, W.; Yuan, H.; Wang, J.; Yao, J.
    Production, purification and characterization of chitosanase produced by Gongronella sp. JG (2008), Lett. Appl. Microbiol., 46, 49-54.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.132 synthesis optimization of production conditions. In the optimized medium, strain JG produces 0.8 mmol per min and l of enzymic activity in 72 h Gongronella sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 Ag+ 1 mM, 3% residaul activity Gongronella sp.
3.2.1.132 EDTA 1 mM, 78% residual activity Gongronella sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.132 Mn2+ 100% stimulation at 1 and 10 mM Gongronella sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 90000
-
 x * 90000, SDS-PAGE Gongronella sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Gongronella sp.
-
 strain JG
-
3.2.1.132 Gongronella sp. JG-2005
-
 strain JG
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132
-
 Gongronella sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 82
-
 pH 4.8, 50°C Gongronella sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O substrate with 85% deacetylation Gongronella sp. chitosan oligosaccharides
-
 ?
3.2.1.132 chitosan + H2O substrate with 85% deacetylation Gongronella sp. JG-2005 chitosan oligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 ? x * 90000, SDS-PAGE Gongronella sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 50
-
-
 Gongronella sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 50
-
 half-life 65 min Gongronella sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 4.6 4.8
-
 Gongronella sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.132 Gongronella sp. isoelectric focusing
-
 5.8