Literature summary extracted from

Mizuno, M.; Koide, A.; Yamamura, A.; Akeboshi, H.; Yoshida, H.; Kamitori, S.; Sakano, Y.; Nishikawa, A.; Tonozuka, T.
Crystal structure of Aspergillus niger isopullulanase, a member of glycoside hydrolase family 49 (2008), J. Mol. Biol., 376, 210-220.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.2.1.57	recombinant IPU produced by Pichia pastoris	Aspergillus niger

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.2.1.57	for crystallization, the enzyme is treated with endoglycosidase Hf, by hanging drop vapor-diffusion method, unliganded and isopanose-complexed forms of IPU, both solved at 1.7 A resolution. Unliganded IPU belongs to space group P212121, which contains two molecules and 1273 water molecules in an asymmetric unit. IPU is composed of domains N and C joined by a short linker, with electron density maps for 11 or 12 N-acetylglucosamine residues per molecule. Domain N consists of 13 beta-strands and forms a beta-sandwich. Domain C, where the active site is located, forms a right-handed beta-helix. Overall conformation of IPU in complex with isopanose is essentially identical with that of unliganded IPU	Aspergillus niger

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.57	Aspergillus niger	O00105	ATCC9642	-
3.2.1.57	Aspergillus niger ATCC 9642	O00105	ATCC9642	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.2.1.57	-	Aspergillus niger

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.2.1.57	pullulan + H2O	Asp353, Asp372 and Asp373 are the catalytic residues of IPU	Aspergillus niger	isopanose + ?	-	?
3.2.1.57	pullulan + H2O	Asp353, Asp372 and Asp373 are the catalytic residues of IPU	Aspergillus niger ATCC 9642	isopanose + ?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.2.1.57	IPU	-	Aspergillus niger
3.2.1.57	pullulan 4-glucanohydrolase	-	Aspergillus niger

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Release 2023.1 (January 2023)