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Literature summary extracted from

  • Proudfoot, M.; Sanders, S.A.; Singer, A.; Zhang, R.; Brown, G.; Binkowski, A.; Xu, L.; Lukin, J.A.; Murzin, A.G.; Joachimiak, A.; Arrowsmith, C.H.; Edwards, A.M.; Savchenko, A.V.; Yakunin, A.F.
    Biochemical and structural characterization of a novel family of cystathionine beta-synthase domain proteins fused to a Zn ribbon-like domain (2008), J. Mol. Biol., 375, 301-315.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.22 N-terminal cystathionine beta-synthase domain fused to the C-terminal Zn ribbon domain is overexpressed in Escherichia coli Pyrococcus furiosus
4.2.1.22 N-terminal cystathionine beta-synthase domain fused to the C-terminal Zn ribbon domain is overexpressed in Escherichia coli Thermoplasma acidophilum
4.2.1.22 N-terminal cystathionine beta-synthase domain fused to the C-terminal Zn ribbon domain is overexpressed in Escherichia coli Pyrococcus horikoshii
4.2.1.22 N-terminal cystathionine beta-synthase domain fused to the C-terminal Zn ribbon domain is overexpressed in Escherichia coli Thermoplasma volcanium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.22 crystal structures of both mercury- and iron-bound TA0289 (1.5–2.0 A resolution) reveals a dimeric protein whose intersubunit contacts are formed exclusively by the alpha-helices of two cystathionine beta-synthase subdomains, whereas the C-terminal domain has a classical Zn ribbon planar architecture Thermoplasma acidophilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.22 Ca2+ contains 0.38 Ca atoms/subunit Pyrococcus furiosus
4.2.1.22 Fe contains 0.13 Fe atoms/subunit Thermoplasma volcanium
4.2.1.22 Fe contains 0.67 Fe atoms/subunit, in minimal medium Thermoplasma acidophilum
4.2.1.22 Fe2+ contains 0.16 Fe atoms/subunit Pyrococcus furiosus
4.2.1.22 Zn contains 0.17 Zn atoms/subunit, in minimal medium Thermoplasma acidophilum
4.2.1.22 Zn contains 0.25 Zn atoms/subunit Thermoplasma volcanium
4.2.1.22 Zn2+ contains 0.35 Zn atoms/subunit Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.22 19700
-
 2 * 19700, calculated from sequence Thermoplasma volcanium
4.2.1.22 20200
-
 3 * 20200, calculated from sequence Pyrococcus furiosus
4.2.1.22 20300
-
 2 * 20300, calculated from sequence Thermoplasma acidophilum
4.2.1.22 36800
-
 gel filtration Thermoplasma volcanium
4.2.1.22 38000
-
 gel filtration Thermoplasma acidophilum
4.2.1.22 62800
-
 gel filtration Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.22 Pyrococcus furiosus
-
-
-
4.2.1.22 Pyrococcus horikoshii
-
-
-
4.2.1.22 Thermoplasma acidophilum
-
-
-
4.2.1.22 Thermoplasma volcanium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.22 N-terminal cystathionine beta-synthase domain fused to the C-terminal Zn ribbon domain overexpressed in Escherichia coli Pyrococcus furiosus
4.2.1.22 N-terminal cystathionine beta-synthase domain fused to the C-terminal Zn ribbon domain overexpressed in Escherichia coli Thermoplasma acidophilum
4.2.1.22 N-terminal cystathionine beta-synthase domain fused to the C-terminal Zn ribbon domain overexpressed in Escherichia coli Pyrococcus horikoshii
4.2.1.22 N-terminal cystathionine beta-synthase domain fused to the C-terminal Zn ribbon domain overexpressed in Escherichia coli Thermoplasma volcanium

Subunits

EC Number Subunits Comment Organism
4.2.1.22 dimer 2 * 19700, calculated from sequence Thermoplasma volcanium
4.2.1.22 dimer 2 * 20300, calculated from sequence Thermoplasma acidophilum
4.2.1.22 trimer 3 * 20200, calculated from sequence Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
4.2.1.22 PF1953
-
 Pyrococcus furiosus
4.2.1.22 PH0267
-
 Pyrococcus horikoshii
4.2.1.22 TA0289
-
 Thermoplasma acidophilum
4.2.1.22 TV1335
-
 Thermoplasma volcanium