Literature summary extracted from
Pan, Y.H.; Bahnson, B.J.
Structural basis for bile salt inhibition of pancreatic phospholipase A2 (2007), J. Mol. Biol., 369, 439-450.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
3.1.1.4 |
cholate |
bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview |
Sus scrofa |
|
3.1.1.4 |
glycochenodeoxycholate |
bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview |
Sus scrofa |
|
3.1.1.4 |
glycocholate |
bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview |
Sus scrofa |
|
3.1.1.4 |
taurochenodeoxycholate |
bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview |
Sus scrofa |
|
3.1.1.4 |
taurocholate |
bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview |
Sus scrofa |
|
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.1.1.4 |
pancreatic PLA2 in complex with five different naturally occurring bile salts, i.e. cholate, taurocholate, glycocholate, glycochenodeoxycholate, and taurochenodeoxycholate, in presence or absence of active-site-directed tetrahedral mimic MJ33, hanging drop vapour diffusion method, 15 mg/ml protein solution with 10 mM CaCl2, 3 mM MJ33, and 3 mM bile salt, is mixed with an equal volume of 0.001 ml of cyrstallization solution containing 0.2 M CaCl2, 0.1 M HEPES, pH 7.5, and 28% v/v PEG 400 or 0.1 M sodium cacodylate, pH 6.5, and 1.4 M sodium acetate, 1 week to 2 months, X-ray diffraction structure determination and analysis at 1.85-2.20 A resolution, modeling |
Sus scrofa |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.1.1.4 |
cholate |
bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview |
Sus scrofa |
|
3.1.1.4 |
glycochenodeoxycholate |
bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview |
Sus scrofa |
|
3.1.1.4 |
glycocholate |
bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview |
Sus scrofa |
|
3.1.1.4 |
MJ33 |
i.e. 1-hexadecyl-3-(trifluoroethyl)-rac-glycero-2-phosphomethanol, an active-site-directed tetrahedral mimic |
Sus scrofa |
|
3.1.1.4 |
taurochenodeoxycholate |
bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview |
Sus scrofa |
|
3.1.1.4 |
taurocholate |
bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview |
Sus scrofa |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.1.1.4 |
additional information |
Sus scrofa |
bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview |
? |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.1.4 |
Sus scrofa |
P00592 |
- |
- |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
3.1.1.4 |
pancreas |
- |
Sus scrofa |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.1.4 |
additional information |
bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview |
Sus scrofa |
? |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.1.4 |
pancreatic phospholipase A2 |
- |
Sus scrofa |