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Literature summary extracted from

  • Yamada, S.; Akiyama, S.; Sugimoto, H.; Kumita, H.; Ito, K.; Fujisawa, T.; Nakamura, H.; Shiro, Y.
    The signaling pathway in histidine kinase and the response regulator complex revealed by X-ray crystallography and solution scattering (2006), J. Mol. Biol., 362, 123-139.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.13.3 additional information responsible role of the response regulator TrrA for the feedback regulation of sensing and/or kinase activities of ThkA Thermotoga maritima

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.13.3 expressed in Escherichia coli BL21 (DE3) CodonPlus RIL-X Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.13.3 ThkA complexed with the response regulator TrrA, by the batch method, X-ray crystallography at a resolution of 4.2 A, 2fold symmetry, two monomeric TrrAs bind to the ThkA dimer Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
2.7.13.3 additional information delta408ThkA, includes the PAS-sensor, the DHp and CA domains, and delta 517ThkA without the PAS-sensor domain, both are fully active in the autophosphorylation reaction Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.13.3 51000
-
 delta517ThkA, experimental estimates Thermotoga maritima
2.7.13.3 76000
-
 delta408ThkA, experimental estimates Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
2.7.13.3 Thermotoga maritima Q9X180
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.13.3 by gel filtration Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.13.3 additional information autophosphorylation activity Thermotoga maritima ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.13.3 dimer crystallography Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
2.7.13.3 histidine kinase
-
 Thermotoga maritima
2.7.13.3 ThkA
-
 Thermotoga maritima