EC Number | Application | Comment | Organism |
---|---|---|---|
2.1.1.148 | drug development | unexpected observation that NADP+ competes with both FAD and substrate for the binding site in ThyX and displaces both molecules from the active site, opens avenues for the design of tight-binding inhibitors of ThyX enzymes from a variety of organisms | Mycobacterium tuberculosis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.148 | I65M/L175M double mutant expressed in Escherichia coli BL21 (DE3) pLysS strain as C-terminal His6-tagged protein | Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.1.1.148 | I65M/L175M double mutant in the presence of FAD and BrdUMP, by the sitting-drop vapor-diffusion method, to 2.0 A resolution | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.148 | I65M/L175M | produces active ThyX enzyme | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.148 | Mycobacterium tuberculosis | P9WG57 | - |
- |
2.1.1.148 | Mycobacterium tuberculosis H37Rv | P9WG57 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.148 | - |
Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.148 | 5,10-methylenetetrahydrofolate + BrdUMP + FADH2 | - |
Mycobacterium tuberculosis | ? | - |
? | |
2.1.1.148 | 5,10-methylenetetrahydrofolate + BrdUMP + FADH2 | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.148 | tetramer | - |
Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.148 | flavin dependent thymidylate synthase | - |
Mycobacterium tuberculosis |
2.1.1.148 | ThyX | - |
Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.148 | FAD | - |
Mycobacterium tuberculosis | |
2.1.1.148 | NADP+ | expulsion of the cofactor FAD by NADP+, no anticipated ThyX-FAD-BrdUMP-NADP+ quaternary complex, but a ThyX-NADP+ binary complex | Mycobacterium tuberculosis |