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Literature summary extracted from

  • Sampathkumar, P.; Turley, S.; Sibley, C.H.; Hol, W.G.
    NADP+ expels both the co-factor and a substrate analog from the Mycobacterium tuberculosis ThyX active site: opportunities for anti-bacterial drug design (2006), J. Mol. Biol., 360, 1-6.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.148 drug development unexpected observation that NADP+ competes with both FAD and substrate for the binding site in ThyX and displaces both molecules from the active site, opens avenues for the design of tight-binding inhibitors of ThyX enzymes from a variety of organisms Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.148 I65M/L175M double mutant expressed in Escherichia coli BL21 (DE3) pLysS strain as C-terminal His6-tagged protein Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.148 I65M/L175M double mutant in the presence of FAD and BrdUMP, by the sitting-drop vapor-diffusion method, to 2.0 A resolution Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.148 I65M/L175M produces active ThyX enzyme Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.148 Mycobacterium tuberculosis P9WG57
-
-
2.1.1.148 Mycobacterium tuberculosis H37Rv P9WG57
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.148
-
Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.148 5,10-methylenetetrahydrofolate + BrdUMP + FADH2
-
Mycobacterium tuberculosis ?
-
?
2.1.1.148 5,10-methylenetetrahydrofolate + BrdUMP + FADH2
-
Mycobacterium tuberculosis H37Rv ?
-
?

Subunits

EC Number Subunits Comment Organism
2.1.1.148 tetramer
-
Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.1.1.148 flavin dependent thymidylate synthase
-
Mycobacterium tuberculosis
2.1.1.148 ThyX
-
Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.148 FAD
-
Mycobacterium tuberculosis
2.1.1.148 NADP+ expulsion of the cofactor FAD by NADP+, no anticipated ThyX-FAD-BrdUMP-NADP+ quaternary complex, but a ThyX-NADP+ binary complex Mycobacterium tuberculosis