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Literature summary extracted from

  • Lee, K.W.; Bae, H.A.; Lee, Y.H.
    Molecular cloning and functional expression of esf gene encoding enantioselective lipase from Serratia marcescens ES-2 for kinetic resolution of optically active (S)-flurbiprofen (2007), J. Microbiol. Biotechnol., 17, 74-80.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 additional information Triton X-207 induces and enhances enzyme secretion from the overexpressing Serratia marcescens strain best at 3.0%, effect of surfactants on secretion of enantioselective Esf lipase in transformant strain BESF overexpressing the esf gene Serratia marcescens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 gene esf, construction and screening of a genetic library, DNA and amino acid sequence determination and analysis, reintroduction, overexpression in, and secretion from the transformed parent strain ES-2, i.e. strain BESF, expression as His-tagged enzyme in Escherichia coli Serratia marcescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular enzyme secretion, the Esf lipase does not possess an N-terminal signal sequence commonly required for the secretion of lipase, but it contains an extreme C-terminal motif consisting of a hydrophobic five-residue sequence that can be recognized by an ABC-transporter system correlated to a signal peptide-independent mechanism for the secretion of protein in Gram-negative bacteria Serratia marcescens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Ca2+ activates 4.6fold at 10 mM, the Esf lipase contains a nine-residue GGXGXDXXX sequence motif in the upstream region of the C-terminal motif, known to form a parallel beta-roll structure binding Ca2+ ions Serratia marcescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 64978
-
 x * 64978, sequence calculation, x * 65000, wild-type enzyme, SDS-PAGE, x * 69000, recombinant His-tagged enzyme Serratia marcescens
3.1.1.3 65000
-
 x * 64978, sequence calculation, x * 65000, wild-type enzyme, SDS-PAGE, x * 69000, recombinant His-tagged enzyme Serratia marcescens
3.1.1.3 69000
-
 x * 64978, sequence calculation, x * 65000, wild-type enzyme, SDS-PAGE, x * 69000, recombinant His-tagged enzyme Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Serratia marcescens Q0MVP2 gene esf
-
3.1.1.3 Serratia marcescens ES-2 Q0MVP2 gene esf
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 native secreted enantioselective lipase 12.1fold from culture supernatant by ultrafiltration, hydrophobic interaction and anion exchange chromatography Serratia marcescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 additional information
-
-
 Serratia marcescens
3.1.1.3 58.4
-
 purified Esf lipase Serratia marcescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 (R,S)-flurbiprofen ethyl ester + H2O high enantiomeric excess and conversion yield of 98% and 48%, respectively Serratia marcescens (S)-flurbiprofen + ethanol
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O
-
 Serratia marcescens 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O
-
 Serratia marcescens ES-2 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl caprate + H2O high activity Serratia marcescens 4-nitrophenol + caprate
-
 ?
3.1.1.3 4-nitrophenyl caproate + H2O high activity Serratia marcescens 4-nitrophenol + caproate
-
 ?
3.1.1.3 4-nitrophenyl caproate + H2O high activity Serratia marcescens ES-2 4-nitrophenol + caproate
-
 ?
3.1.1.3 4-nitrophenyl caprylate + H2O best 4-nitrophenyl ester substrate Serratia marcescens 4-nitrophenol + caprylate
-
 ?
3.1.1.3 4-nitrophenyl caprylate + H2O best 4-nitrophenyl ester substrate Serratia marcescens ES-2 4-nitrophenol + caprylate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Serratia marcescens 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl myristate + H2O
-
 Serratia marcescens 4-nitrophenol + myristate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O low activity Serratia marcescens 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl propionate + H2O low activity Serratia marcescens 4-nitrophenol + propionate
-
 ?
3.1.1.3 4-nitrophenyl propionate + H2O low activity Serratia marcescens ES-2 4-nitrophenol + propionate
-
 ?
3.1.1.3 additional information substrate specificity, no or poor activity with tributyrin, triacetin, tripropionin, tristearin, 4-nitrophenyl stearate, and 4-nitrophenyl acetate, the lipase shows preferential substrate specificity toward the medium-chain-length fatty acids, overview Serratia marcescens ?
-
 ?
3.1.1.3 additional information substrate specificity, no or poor activity with tributyrin, triacetin, tripropionin, tristearin, 4-nitrophenyl stearate, and 4-nitrophenyl acetate, the lipase shows preferential substrate specificity toward the medium-chain-length fatty acids, overview Serratia marcescens ES-2 ?
-
 ?
3.1.1.3 tricaprin + H2O best triacylglyceride substrate Serratia marcescens dicaprin + caprate
-
 ?
3.1.1.3 tricaproin + H2O
-
 Serratia marcescens dicaproin + caproate
-
 ?
3.1.1.3 tricaprylin + H2O
-
 Serratia marcescens dicaprylin + caprylate
-
 ?
3.1.1.3 trilaurin + H2O high activity Serratia marcescens dilaurin + laurate
-
 ?
3.1.1.3 trimyristin + H2O
-
 Serratia marcescens dimyristin + myristate
-
 ?
3.1.1.3 tripalmitin + H2O low activity Serratia marcescens dipalmitin + palmitate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 64978, sequence calculation, x * 65000, wild-type enzyme, SDS-PAGE, x * 69000, recombinant His-tagged enzyme Serratia marcescens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 enantioselective lipase
-
 Serratia marcescens
3.1.1.3 Esf lipase
-
 Serratia marcescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 37
-
-
 Serratia marcescens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 20 80
-
 Serratia marcescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 7
-
-
 Serratia marcescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 4 11
-
 Serratia marcescens