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Literature summary extracted from

  • Bourgois, T.M.; Nguyen, D.V.; Sansen, S.; Rombouts, S.; Belien, T.; Fierens, K.; Raedschelders, G.; Rabijns, A.; Courtin, C.M.; Delcour, J.A.; Van Campenhout, S.; Volckaert, G.
    Targeted molecular engineering of a family 11 endoxylanase to decrease its sensitivity towards Triticum aestivum endoxylanase inhibitor types (2007), J. Biotechnol., 130, 95-105.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.8 food industry improvement of cereal-based industrial processing by endoxylanase enzymes insensitive towards inhibitors Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 expressed in Escherichia coli WK6 cells Aspergillus niger
3.2.1.8 expresssed in Escherichia coli WK6 cells Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.8 D11F/R122D no inhibition by Triticum aestivum xylanase inhibitor-I Aspergillus niger
3.2.1.8 G12 no inhibition by Triticum aestivum xylanase inhibitor-I Aspergillus niger
3.2.1.8 G12K decreased sensitivity to Triticum aestivum xylanase inhibitor-I Aspergillus niger
3.2.1.8 G12W no inhibition by Triticum aestivum xylanase inhibitor-I Aspergillus niger
3.2.1.8 N35D increased sensitivity to Triticum aestivum xylanase inhibitor-I Aspergillus niger
3.2.1.8 Q127E specific activity is lower than 5% compared to the wild type enzyme Aspergillus niger
3.2.1.8 Q127K no inhibition by Triticum aestivum xylanase inhibitor-I Aspergillus niger
3.2.1.8 Q127L specific activity is lower than 5% compared to the wild type enzyme Aspergillus niger
3.2.1.8 R112H wild type comparable sensitivity to TAXI-I Aspergillus niger
3.2.1.8 R112Y no inhibition by Triticum aestivum xylanase inhibitor-I Aspergillus niger
3.2.1.8 W9Y mutant enzyme is insensitive for Triticum aestivum xylanase inhibitor-II Aspergillus niger
3.2.1.8 W9Y/N35D increased sensitivity to Triticum aestivum xylanase inhibitor-I and insensitive for Triticum aestivum xylanase inhibitor-II Aspergillus niger
3.2.1.8 Y174W increased sensitivity to Triticum aestivum xylanase inhibitor-I Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Triticum aestivum endoxylanase inhibitor-I
-
 Bacillus subtilis
3.2.1.8 Triticum aestivum endoxylanase inhibitor-II
-
 Bacillus subtilis
3.2.1.8 Triticum aestivum xylanase inhibitor-I
-
 Aspergillus niger
3.2.1.8 Triticum aestivum xylanase inhibitor-II
-
 Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Aspergillus niger
-
-
-
3.2.1.8 Bacillus subtilis P18429
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 on Ni-NTA column Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 91
-
 mutant Q127E Bacillus subtilis
3.2.1.8 245
-
 mutant Q127L Bacillus subtilis
3.2.1.8 594
-
 mutant R112Y Bacillus subtilis
3.2.1.8 619
-
 mutant Q127K Bacillus subtilis
3.2.1.8 1655
-
 mutant D11F/R122D Bacillus subtilis
3.2.1.8 3707
-
 mutant W9Y Bacillus subtilis
3.2.1.8 3722
-
 mutant R112H Bacillus subtilis
3.2.1.8 4031
-
 mutant N35D Bacillus subtilis
3.2.1.8 4890
-
 mutant G12R Bacillus subtilis
3.2.1.8 5072
-
 mutant G12W Bacillus subtilis
3.2.1.8 5152
-
 mutant W9Y/N35D Bacillus subtilis
3.2.1.8 5234
-
 mutant G12K Bacillus subtilis
3.2.1.8 5779
-
 mutant Y174W Bacillus subtilis
3.2.1.8 5890
-
 wild-type Bacillus subtilis
3.2.1.8 5890
-
 wild type enzyme, at pH 7.0 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 arabinoxylan + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.8 arabinoxylan + H2O
-
 Bacillus subtilis ?
-
 ?
3.2.1.8 birchwood xylan + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.8 birchwood xylan + H2O
-
 Bacillus subtilis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 BSXY
-
 Aspergillus niger
3.2.1.8 BSXY
-
 Bacillus subtilis
3.2.1.8 endoxylanase
-
 Aspergillus niger
3.2.1.8 family 11 endoxylanase
-
 Bacillus subtilis
3.2.1.8 XynA
-
 Aspergillus niger
3.2.1.8 XynA
-
 Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 0.039
-
 birchwood xylan wild-type Bacillus subtilis
3.2.1.8 0.039
-
 birchwood xylan wild type enzyme, at pH 7.0 Aspergillus niger
3.2.1.8 2
-
 birchwood xylan mutant W9Y/N35D Bacillus subtilis
3.2.1.8 8.8
-
 birchwood xylan mutant R112Y Bacillus subtilis
3.2.1.8 14.6
-
 birchwood xylan mutant Q127K Bacillus subtilis
3.2.1.8 21
-
 birchwood xylan mutant Y174W Bacillus subtilis
3.2.1.8 39
-
 birchwood xylan mutant D11F/R122D Bacillus subtilis
3.2.1.8 50.7
-
 birchwood xylan mutant N35D Bacillus subtilis
3.2.1.8 65.4
-
 birchwood xylan mutant G12W Bacillus subtilis
3.2.1.8 71.6
-
 birchwood xylan mutant W9Y/N35D Bacillus subtilis
3.2.1.8 88.7
-
 birchwood xylan mutant W9Y Bacillus subtilis
3.2.1.8 111.2
-
 birchwood xylan mutant Y174W Bacillus subtilis
3.2.1.8 140.1
-
 birchwood xylan wild-type Bacillus subtilis
3.2.1.8 140.1
-
 birchwood xylan wild type enzyme, at pH 7.0 Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 7
-
-
 Aspergillus niger
3.2.1.8 7
-
 for most mutants the pH optimum is around 5.5-6.0 Bacillus subtilis