EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.6 | 3.28 | - |
4-nitrophenyl-2-O-acetyl-beta-D-xylopyranoside | pH 5.5, 45°C | Aspergillus oryzae | |
3.1.1.6 | 6.78 | - |
4-nitrophenyl-4-O-acetyl-beta-D-xylopyranoside | pH 5.5, 45°C | Aspergillus oryzae | |
3.1.1.6 | 9.44 | - |
4-nitrophenyl-3-O-acetyl-beta-D-xylopyranoside | pH 5.5, 45°C | Aspergillus oryzae | |
3.1.1.6 | 16.4 | - |
4-nitrophenyl-5-O-acetyl-alpha-L-arabinofuranoside | pH 5.5, 45°C | Aspergillus oryzae | |
3.1.1.6 | 44.6 | - |
4-nitrophenyl-2-O-acetyl-alpha-L-arabinofuranoside | pH 5.5, 45°C | Aspergillus oryzae | |
3.1.1.73 | 0.016 | - |
4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside | - |
Talaromyces stipitatus | |
3.1.1.73 | 0.028 | - |
4-nitrophenyl 4-O-(E)-feruloyl-alpha-D-xylopyranoside | - |
Talaromyces stipitatus | |
3.1.1.73 | 0.042 | - |
4-nitrophenyl 2-O-(E)-feruloyl-alpha-L-arabinofuranoside | - |
Talaromyces stipitatus | |
3.1.1.73 | 0.053 | - |
4-nitrophenyl 3-O-(E)-feruloyl-alpha-L-arabinofuranoside | - |
Talaromyces stipitatus | |
3.1.1.73 | 0.073 | - |
4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside | - |
Aspergillus niger | |
3.1.1.73 | 0.097 | - |
4-nitrophenyl 2-O-(E)-feruloyl-alpha-D-xylopyranoside | - |
Talaromyces stipitatus | |
3.1.1.73 | 0.123 | - |
4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside | - |
Fusarium oxysporum | |
3.1.1.73 | 0.24 | - |
4-nitrophenyl 4-O-(E)-feruloyl-alpha-D-xylopyranoside | - |
Aspergillus niger | |
3.1.1.73 | 0.25 | - |
4-nitrophenyl 3-O-(E)-feruloyl-alpha-L-arabinofuranoside | - |
Fusarium oxysporum | |
3.1.1.73 | 0.28 | - |
4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside | - |
Fusarium oxysporum | |
3.1.1.73 | 0.54 | - |
4-nitrophenyl 2-O-(E)-feruloyl-alpha-L-arabinofuranoside | - |
Fusarium oxysporum | |
3.1.1.73 | 0.57 | - |
4-nitrophenyl 3-O-(E)-feruloyl-alpha-D-xylopyranoside | - |
Fusarium oxysporum | |
3.1.1.73 | 1.24 | - |
4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside | - |
Aspergillus niger | |
3.1.1.73 | 1.5 | 2 | 4-nitrophenyl 2-O-(E)-feruloyl-alpha-D-xylopyranoside | - |
Aspergillus niger | |
3.1.1.73 | 1.51 | - |
4-nitrophenyl 3-O-(E)-feruloyl-alpha-D-xylopyranoside | - |
Aspergillus niger | |
3.1.1.73 | 2.26 | - |
4-nitrophenyl 2-O-(E)-feruloyl-alpha-D-xylopyranoside | - |
Fusarium oxysporum | |
3.1.1.73 | 3.82 | - |
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside | - |
Aspergillus oryzae | |
3.1.1.73 | 6.78 | - |
4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside | - |
Aspergillus oryzae | |
3.1.1.73 | 6.98 | - |
4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside | - |
Fusarium oxysporum | |
3.1.1.73 | 9.44 | - |
4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside | - |
Aspergillus oryzae | |
3.1.1.73 | 16.4 | - |
4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside | - |
Aspergillus oryzae | |
3.1.1.73 | 17 | - |
4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside | - |
Fusarium oxysporum | |
3.1.1.73 | 44.6 | - |
4-nitrophenyl 2-O-acetyl-alpha-L-arabinofuranoside | - |
Aspergillus oryzae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.6 | 30000 | - |
x * 30000, SDS-PAGE | Aspergillus oryzae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.6 | Aspergillus oryzae | - |
- |
- |
3.1.1.73 | Aspergillus niger | O42807 | - |
- |
3.1.1.73 | Aspergillus oryzae | - |
- |
- |
3.1.1.73 | Fusarium oxysporum | - |
- |
- |
3.1.1.73 | Talaromyces stipitatus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.6 | native enzyme to homogeneity by SDS-PAGE and isoelectric focusing | Aspergillus oryzae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.6 | additional information | - |
- |
Aspergillus oryzae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.6 | 4-nitrophenyl-2-O-acetyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus oryzae | 4-nitrophenyl-alpha-L-arabinofuranoside + acetate | - |
? | |
3.1.1.6 | 4-nitrophenyl-2-O-acetyl-beta-D-xylopyranoside + H2O | preferred substrate | Aspergillus oryzae | 4-nitrophenyl-beta-D-xylopyranoside + acetate | - |
? | |
3.1.1.6 | 4-nitrophenyl-3-O-acetyl-beta-D-xylopyranoside + H2O | - |
Aspergillus oryzae | 4-nitrophenyl-beta-D-xylopyranoside + acetate | - |
? | |
3.1.1.6 | 4-nitrophenyl-4-O-acetyl-beta-D-xylopyranoside + H2O | - |
Aspergillus oryzae | 4-nitrophenyl-beta-D-xylopyranoside + acetate | - |
? | |
3.1.1.6 | 4-nitrophenyl-5-O-acetyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus oryzae | 4-nitrophenyl-alpha-L-arabinofuranoside + acetate | - |
? | |
3.1.1.6 | additional information | the enzyme, feruloyl esterase AoFae, behaves as a non-specific acetyl esterase rather than a feruloyl esterase, with a preference for 2-O-acetyl-beta-D-xylopyranoside, AoFae shows only a very weak activity on feruloyl esters | Aspergillus oryzae | ? | - |
? | |
3.1.1.73 | 4-nitrophenyl 2-O-(E)-feruloyl-alpha-D-xylopyranoside + H2O | - |
Fusarium oxysporum | 4-nitrophenyl-alpha-D-xylopyranoside + ferulate | - |
? | |
3.1.1.73 | 4-nitrophenyl 2-O-(E)-feruloyl-alpha-D-xylopyranoside + H2O | - |
Talaromyces stipitatus | 4-nitrophenyl-alpha-D-xylopyranoside + trans-ferulate | - |
? | |
3.1.1.73 | 4-nitrophenyl 2-O-(E)-feruloyl-alpha-D-xylopyranoside + H2O | - |
Aspergillus niger | 4-nitrophenyl-alpha-D-xylopyranoside + trans-ferulate | - |
? | |
3.1.1.73 | 4-nitrophenyl 2-O-(E)-feruloyl-alpha-L-arabinofuranoside + H2O | - |
Fusarium oxysporum | 4-nitrophenyl-alpha-L-arabinofuranoside + ferulate | - |
? | |
3.1.1.73 | 4-nitrophenyl 2-O-(E)-feruloyl-alpha-L-arabinofuranoside + H2O | - |
Talaromyces stipitatus | 4-nitrophenyl-alpha-L-arabinofuranoside + trans-ferulate | - |
? | |
3.1.1.73 | 4-nitrophenyl 2-O-acetyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus oryzae | 4-nitrophenyl-alpha-L-arabinofuranoside + acetate | - |
? | |
3.1.1.73 | 4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O | - |
Aspergillus oryzae | 4-nitrophenyl-beta-D-xylopyranoside + acetate | - |
? | |
3.1.1.73 | 4-nitrophenyl 3-O-(E)-feruloyl-alpha-D-xylopyranoside + H2O | - |
Fusarium oxysporum | 4-nitrophenyl-alpha-D-xylopyranoside + ferulate | - |
? | |
3.1.1.73 | 4-nitrophenyl 3-O-(E)-feruloyl-alpha-D-xylopyranoside + H2O | - |
Aspergillus niger | 4-nitrophenyl-alpha-D-xylopyranoside + trans-ferulate | - |
? | |
3.1.1.73 | 4-nitrophenyl 3-O-(E)-feruloyl-alpha-L-arabinofuranoside + H2O | - |
Fusarium oxysporum | 4-nitrophenyl-alpha-L-arabinofuranoside + ferulate | - |
? | |
3.1.1.73 | 4-nitrophenyl 3-O-(E)-feruloyl-alpha-L-arabinofuranoside + H2O | - |
Talaromyces stipitatus | 4-nitrophenyl alpha-L-arabinofuranoside + ferulate | - |
? | |
3.1.1.73 | 4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside + H2O | - |
Aspergillus oryzae | 4-nitrophenyl-beta-D-xylopyranoside + acetate | - |
? | |
3.1.1.73 | 4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside + H2O | - |
Fusarium oxysporum | ? | - |
? | |
3.1.1.73 | 4-nitrophenyl 4-O-(E)-feruloyl-alpha-D-xylopyranoside + H2O | - |
Talaromyces stipitatus | 4-nitrophenyl-alpha-D-xylopyranoside + trans-ferulate | - |
? | |
3.1.1.73 | 4-nitrophenyl 4-O-(E)-feruloyl-alpha-D-xylopyranoside + H2O | - |
Aspergillus niger | 4-nitrophenyl-alpha-D-xylopyranoside + trans-ferulate | - |
? | |
3.1.1.73 | 4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside + H2O | - |
Aspergillus oryzae | 4-nitrophenyl-beta-D-xylopyranoside + acetate | - |
? | |
3.1.1.73 | 4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside + H2O | - |
Fusarium oxysporum | 4-nitrophenyl-beta-D-xylopyranoside + acetate | - |
? | |
3.1.1.73 | 4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside + H2O | - |
Fusarium oxysporum | 4-nitrophenyl-alpha-L-arabinofuranoside + ferulate | - |
? | |
3.1.1.73 | 4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside + H2O | - |
Fusarium oxysporum | 4-nitrophenyl-alpha-L-arabinofuranoside + trans-ferulate | - |
? | |
3.1.1.73 | 4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside + H2O | - |
Talaromyces stipitatus | 4-nitrophenyl alpha-L-arabinofuranoside + ferulate | - |
? | |
3.1.1.73 | 4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | 4-nitrophenyl alpha-L-arabinofuranoside + ferulate | - |
? | |
3.1.1.73 | 4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus oryzae | 4-nitrophenyl-alpha-L-arabinofuranoside + acetate | - |
? | |
3.1.1.73 | 4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside + H2O | - |
Fusarium oxysporum | 4-nitrophenyl-alpha-L-arabinofuranoside + acetate | - |
? | |
3.1.1.73 | 4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside + H2O | the enzyme is specialized for deferuloylation of primary hydroxyl groups, with a very strong preference for hydrolyzing 5-O-feruloyl-alpha-L-arabinofuranoside | Aspergillus niger | 4-nitrophenyl-alpha-L-arabinofuranoside + acetate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.6 | ? | x * 30000, SDS-PAGE | Aspergillus oryzae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.6 | More | the enzyme belongs to the carbohydrate esterase family 1 | Aspergillus oryzae |
3.1.1.6 | non-specific acetyl esterase | - |
Aspergillus oryzae |
3.1.1.73 | AnFaeA | - |
Aspergillus niger |
3.1.1.73 | AoFaeC | - |
Aspergillus oryzae |
3.1.1.73 | FoFaeI | - |
Fusarium oxysporum |
3.1.1.73 | FoFaeII | - |
Fusarium oxysporum |
3.1.1.73 | TsFaeC | - |
Talaromyces stipitatus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.6 | 50 | - |
- |
Aspergillus oryzae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.6 | 0.15 | - |
4-nitrophenyl-2-O-acetyl-alpha-L-arabinofuranoside | pH 5.5, 45°C | Aspergillus oryzae | |
3.1.1.6 | 0.91 | - |
4-nitrophenyl-4-O-acetyl-beta-D-xylopyranoside | pH 5.5, 45°C | Aspergillus oryzae | |
3.1.1.6 | 1.77 | - |
4-nitrophenyl-5-O-acetyl-alpha-L-arabinofuranoside | pH 5.5, 45°C | Aspergillus oryzae | |
3.1.1.6 | 1.9 | - |
4-nitrophenyl-3-O-acetyl-beta-D-xylopyranoside | pH 5.5, 45°C | Aspergillus oryzae | |
3.1.1.6 | 3.23 | - |
4-nitrophenyl-2-O-acetyl-beta-D-xylopyranoside | pH 5.5, 45°C | Aspergillus oryzae | |
3.1.1.73 | 0.03 | 0.55 | 4-nitrophenyl 2-O-(E)-feruloyl-alpha-L-arabinofuranoside | - |
Fusarium oxysporum | |
3.1.1.73 | 0.15 | - |
4-nitrophenyl 2-O-acetyl-alpha-L-arabinofuranoside | - |
Aspergillus oryzae | |
3.1.1.73 | 0.24 | - |
4-nitrophenyl 4-O-(E)-feruloyl-alpha-D-xylopyranoside | - |
Aspergillus niger | |
3.1.1.73 | 0.7 | - |
4-nitrophenyl 3-O-(E)-feruloyl-alpha-D-xylopyranoside | - |
Aspergillus niger | |
3.1.1.73 | 0.81 | - |
4-nitrophenyl 3-O-(E)-feruloyl-alpha-L-arabinofuranoside | - |
Fusarium oxysporum | |
3.1.1.73 | 0.86 | - |
4-nitrophenyl 3-O-(E)-feruloyl-alpha-D-xylopyranoside | - |
Fusarium oxysporum | |
3.1.1.73 | 0.89 | - |
4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside | - |
Fusarium oxysporum | |
3.1.1.73 | 0.91 | - |
4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside | - |
Aspergillus oryzae | |
3.1.1.73 | 1.17 | - |
4-nitrophenyl 2-O-(E)-feruloyl-alpha-D-xylopyranoside | - |
Aspergillus niger | |
3.1.1.73 | 1.77 | - |
4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside | - |
Aspergillus oryzae | |
3.1.1.73 | 1.9 | - |
4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside | - |
Aspergillus oryzae | |
3.1.1.73 | 2.33 | - |
4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside | - |
Aspergillus niger | |
3.1.1.73 | 2.73 | - |
4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside | - |
Fusarium oxysporum | |
3.1.1.73 | 3.23 | - |
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside | - |
Aspergillus oryzae | |
3.1.1.73 | 3.66 | - |
4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside | - |
Fusarium oxysporum | |
3.1.1.73 | 5.2 | - |
4-nitrophenyl 2-O-(E)-feruloyl-alpha-D-xylopyranoside | - |
Fusarium oxysporum | |
3.1.1.73 | 6.37 | - |
4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside | - |
Fusarium oxysporum | |
3.1.1.73 | 7.05 | - |
4-nitrophenyl 2-O-(E)-feruloyl-alpha-L-arabinofuranoside | - |
Fusarium oxysporum | |
3.1.1.73 | 17.9 | - |
4-nitrophenyl 4-O-(E)-feruloyl-alpha-D-xylopyranoside | - |
Talaromyces stipitatus | |
3.1.1.73 | 22.5 | - |
4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside | - |
Talaromyces stipitatus | |
3.1.1.73 | 34.2 | - |
4-nitrophenyl 3-O-(E)-feruloyl-alpha-L-arabinofuranoside | - |
Talaromyces stipitatus | |
3.1.1.73 | 38.9 | - |
4-nitrophenyl 2-O-(E)-feruloyl-alpha-L-arabinofuranoside | - |
Talaromyces stipitatus | |
3.1.1.73 | 39.7 | - |
4-nitrophenyl 2-O-(E)-feruloyl-alpha-D-xylopyranoside | - |
Talaromyces stipitatus | |
3.1.1.73 | 175 | - |
4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside | - |
Aspergillus niger |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.6 | 4.5 | 6 | - |
Aspergillus oryzae |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.1.6 | Aspergillus oryzae | isoelectric focusing | - |
3.6 |