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Literature summary extracted from

  • Ootsuka, S.; Saga, N.; Suzuki, K.; Inoue, A.; Ojima, T.
    Isolation and cloning of an endo-beta-1,4-mannanase from Pacific abalone Haliotis discus hannai (2006), J. Biotechnol., 125, 269-280.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.78 2-mercaptoethanol 1 mM, 11% increase in activity Haliotis discus hannai
3.2.1.78 Ca2+ 1 mM, 20% increase in activity Haliotis discus hannai

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.78 Ag+ 1 mM, complete loss of activity Haliotis discus hannai
3.2.1.78 Co2+ 1 mM, 50% loss of activity Haliotis discus hannai
3.2.1.78 Cu2+ 1 mM, 49% loss of activity Haliotis discus hannai
3.2.1.78 dithiothreitol 1 mM, 25% loss of activity Haliotis discus hannai
3.2.1.78 Fe2+ 1 mM, 40% loss of activity Haliotis discus hannai

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.78 39000
-
 x * 39000, SDS-PAGE, x * 39627, calculated for mature protein Haliotis discus hannai
3.2.1.78 39627
-
 x * 39000, SDS-PAGE, x * 39627, calculated for mature protein Haliotis discus hannai

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Haliotis discus hannai
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.78 proteolytic modification sequence contains a signal peptide of 17 amino acids Haliotis discus hannai

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78 from digestive fluid Haliotis discus hannai

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.78 digestive juice
-
 Haliotis discus hannai
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 4.8
-
 pH 7.0, 30°C, substrate beta-1,4-mannan Haliotis discus hannai
3.2.1.78 9.6
-
 pH 7.0, 30°C, substrate glucomannan Haliotis discus hannai
3.2.1.78 11.48
-
 pH 7.0, 30°C, substrate galactomannan Haliotis discus hannai

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 beta-1,4-mannan + H2O
-
 Haliotis discus hannai beta-1,4-mannotriose + beta-1,4-mannotetraose
-
 ?
3.2.1.78 galactomannan + H2O source of substrate locust bean gum Haliotis discus hannai galactosyl-mannosyl oligosaccharides
-
 ?
3.2.1.78 glucomannan + H2O source of substrate konjac Haliotis discus hannai glucosyl-mannosyl oligosaccharides
-
 ?
3.2.1.78 additional information enzyme degrades either beta-1,4-mannan or beta-1,4-mannooligosaccharides to mannotriose and mannotetraose. Enzyme disperses the fronds of the red alga Porphyra yezoensis into cell masses consisting of 10-20 cells that are available for cell engineering Haliotis discus hannai ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 39000, SDS-PAGE, x * 39627, calculated for mature protein Haliotis discus hannai

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 45
-
-
 Haliotis discus hannai

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 40
-
 30 min, stable Haliotis discus hannai

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 7.5
-
-
 Haliotis discus hannai