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Literature summary extracted from

  • Maqbool, Q.U.; Johri, S.; Rasool, S.; Riyaz-ul-Hassan, S.; Verma, V.; Nargotra, A.; Koul, S.; Qazi, G.N.
    Molecular cloning of carboxylesterase gene and biochemical characterization of encoded protein from Bacillus subtilis (RRL BB1) (2006), J. Biotechnol., 125, 1-10.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 expression in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.1 alignment studies show that enzyme has an alpha/beta hydrolase fold with catalytic triad formed by S190, E305, and H394 Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 Ag+ 1 mM, more than 70% loss of activity Bacillus subtilis
3.1.1.1 ascorbic acid 1 mM, 40% residual activity Bacillus subtilis
3.1.1.1 Cu2+ 1 mM, more than 70% loss of activity Bacillus subtilis
3.1.1.1 Fe2+ 1 mM, more than 70% loss of activity Bacillus subtilis
3.1.1.1 Fe3+ 1 mM, more than 70% loss of activity Bacillus subtilis
3.1.1.1 additional information not inhibitory: detergents CHAPS, Tween-20, Triton X-100 at 1%, or EDTA, dithiothreitol, 2-mercaptoethanol Bacillus subtilis
3.1.1.1 sodium dodecylsulfate 0.1%, strong inhibition Bacillus subtilis
3.1.1.1 Zn2+ 1 mM, more than 70% loss of activity Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.045
-
4-nitrophenyl butanoate pH 7.5, 22°C Bacillus subtilis
3.1.1.1 0.077
-
4-nitrophenyl caproate pH 7.5, 22°C Bacillus subtilis
3.1.1.1 0.196
-
Triacetin pH 7.5, 22°C Bacillus subtilis
3.1.1.1 2.22
-
tributyrin pH 7.5, 22°C Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 52000
-
x * 52000, calculated Bacillus subtilis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.1 Acetone 20% v/v, 70-80% residual activity, 60% v/v, 45% residual activity Bacillus subtilis
3.1.1.1 Ethanol 20% v/v, 70-80% residual activity, 60% v/v, almost complete inactivation Bacillus subtilis
3.1.1.1 Methanol 20% v/v, 70-80% residual activity, 60% v/v, 45% residual activity Bacillus subtilis
3.1.1.1 propanol 20% v/v, 70-80% residual activity, 60% v/v, almost complete inactivation Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Bacillus subtilis Q4PNS3 enantioselective enzyme estBB1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1
-
Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 1735
-
pH 7.5, 22°C Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl acetate + H2O
-
Bacillus subtilis 4-nitrophenol + acetate 92% of the activity with 4-nitrophenyl butanoate ?
3.1.1.1 4-nitrophenyl butanoate + H2O
-
Bacillus subtilis 4-nitrophenol + butanoate
-
?
3.1.1.1 4-nitrophenyl caproate + H2O
-
Bacillus subtilis 4-nitrophenol + caproate 65% of the activity with 4-nitrophenyl butanoate ?
3.1.1.1 4-nitrophenyl caprylate + H2O
-
Bacillus subtilis 4-nitrophenol + caprylate 45% of the activity with 4-nitrophenyl butanoate ?
3.1.1.1 4-nitrophenyl laurate + H2O
-
Bacillus subtilis 4-nitrophenol + laurate 30-35% of the activity with 4-nitrophenyl butanoate ?
3.1.1.1 ethyl 2-hydroxy-4-phenylbutanoate + H2O
-
Bacillus subtilis 2-hydroxy-4-phenylbutanoate + ethanol enantiopreference for (S)-isomer with enantiomeric excess of 80% ?
3.1.1.1 additional information no substrate: triglycerides with chain length of C8 to C18 Bacillus subtilis ?
-
?
3.1.1.1 triacetin + H2O about 65% of the activity with tributyrin Bacillus subtilis acetic acid + glycerol
-
?
3.1.1.1 tributyrin + H2O
-
Bacillus subtilis butanoate + glycerol
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 ? x * 52000, calculated Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 estBB1
-
Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 45
-
sharp decrease in stability above Bacillus subtilis
3.1.1.1 50
-
30 min, complete loss of activity Bacillus subtilis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.1 Bacillus subtilis calculated
-
5.2