EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.22.70 | mutant enzymes expressed in Escherichia coli | Staphylococcus aureus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.22.70 | D170A | Tm is 1.7°C higher than the Tm-value of wild-type enzyme. No change in kcat/Km compared to wild-type value | Staphylococcus aureus |
3.4.22.70 | E171A | Tm is 1.8°C lower than the Tm-value of wild-type enzyme. kcat/Km is 5.4fold lower than wild-type value | Staphylococcus aureus |
3.4.22.70 | G167A | Tm is 0.5°C lower than the Tm-value of wild-type enzyme. No change in kcat/KM compared to wild-type enzyme | Staphylococcus aureus |
3.4.22.70 | L169A | Tm is 1.8°C lower than the Tm-value of wild-type enzyme. kcat/Km is 93fold lower than wild-type value | Staphylococcus aureus |
3.4.22.70 | Q172A | Tm is 1.2°C lower than the Tm-value of wild-type enzyme. kcat/Km is 1.4fold lower than wild-type value | Staphylococcus aureus |
3.4.22.70 | R197A | Tm is 2.9°C lower than the Tm-value of wild-type enzyme. kcat/Km is 960fold lower than wild-type value | Staphylococcus aureus |
3.4.22.70 | R197Cit | generation of a semi-synthetic SrtA in which Arg197 is replaced with citrulline, a nonionizable analog. This change results in less than a 3fold decrease in kcat/KM, indicating that Arg197 utilizes a hydrogen bond, rather than an electrostatic interaction. Tm is 0.3°C higher than the Tm-value of wild-type enzyme | Staphylococcus aureus |
3.4.22.70 | R197Cit | kcat/Km is 5.9fold lower than wild-type value | Staphylococcus aureus |
3.4.22.70 | R197K | Tm is 2°C lower than the Tm-value of wild-type enzyme. kcat/Km is 690fold lower than wild-type value | Staphylococcus aureus |
3.4.22.70 | V168A | Tm is 1.7°C lower than the Tm-value of wild-type enzyme. kcat/Km is 5.5fold lower than wild-type value | Staphylococcus aureus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.70 | 4.69 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme R197A | Staphylococcus aureus | |
3.4.22.70 | 6.56 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme V168A | Staphylococcus aureus | |
3.4.22.70 | 6.74 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme E171A | Staphylococcus aureus | |
3.4.22.70 | 8.13 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme D170A | Staphylococcus aureus | |
3.4.22.70 | 8.7 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme G167A | Staphylococcus aureus | |
3.4.22.70 | 8.76 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, wild-type enzyme | Staphylococcus aureus | |
3.4.22.70 | 9.14 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme L169A | Staphylococcus aureus | |
3.4.22.70 | 10.4 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme R197K | Staphylococcus aureus | |
3.4.22.70 | 12.7 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme Q172A | Staphylococcus aureus | |
3.4.22.70 | 13.7 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme R197Cit | Staphylococcus aureus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.70 | Staphylococcus aureus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.22.70 | - |
Staphylococcus aureus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.70 | Abz-LPETGG-Dap(Dnp)-NH2 + GGGGG | - |
Staphylococcus aureus | Abz-LPETGGGGGG + Gly-Dap(Dnp)-NH2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.22.70 | SrtA | - |
Staphylococcus aureus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.70 | 0.000628 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme R197A | Staphylococcus aureus | |
3.4.22.70 | 0.0019 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme R197K | Staphylococcus aureus | |
3.4.22.70 | 0.0123 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme L169A | Staphylococcus aureus | |
3.4.22.70 | 0.15 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme V168A | Staphylococcus aureus | |
3.4.22.70 | 0.16 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme E171A | Staphylococcus aureus | |
3.4.22.70 | 0.29 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme R197Cit | Staphylococcus aureus | |
3.4.22.70 | 1.09 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme D170A | Staphylococcus aureus | |
3.4.22.70 | 1.1 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, wild-type enzyme | Staphylococcus aureus | |
3.4.22.70 | 1.13 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme G167A | Staphylococcus aureus | |
3.4.22.70 | 1.13 | - |
aminobenzoyl-LPETGG-diaminopropionic acid(dinitrophenol)-NH2 | 37°C, pH 7.5, mutant enzyme Q172A | Staphylococcus aureus |