Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bentley, M.L.; Gaweska, H.; Kielec, J.M.; McCafferty, D.G.
    Engineering the substrate specificity of Staphylococcus aureus sortase A. The beta6/beta7 loop from SrtB confers NPQTN recognition to SrtA (2007), J. Biol. Chem., 282, 6571-6581.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.70 N-terminally His6-tagged SrtADELTAN24 is expressed in Escherichia coli BL21 Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.70 additional information marked changes in the specificity profile of SrtA are obtained by replacing the beta6/beta7 loop in SrtA with the corresponding domain from SrtB. The chimeric beta6/beta7 loop swap enzyme (SrtLS) conferrs the ability to acylate NPQTN-containing substrates, with a kcat/Kmapp of 0.0062 /M * s. This enzyme is unable to perform the transpeptidation stage of the reaction, suggesting that additional domains are required for transpeptidation to occur. The overall catalytic specificity profile (kcat/Kmapp (NPQTN)/kcat/Kmapp (LPETG)) of SrtLS is altered 700000fold from SrtA. These results indicate that the beta6/beta7 loop is an important site for substrate recognition in sortases Staphylococcus aureus
3.4.22.71 additional information marked changes in the specificity profile of SrtA are obtained by replacing the beta6/beta7 loop in SrtA with the corresponding domain from SrtB Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.70 Staphylococcus aureus
-
-
-
3.4.22.71 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.70 N-terminally His6-tagged SrtADELTAN24 expressed in Escherichia coli BL21 Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.70 Abz-KVENPQTNAGT-Dap(Dnp)-NH2 + GGGGG
-
 Staphylococcus aureus Abz-KVENPQTGGGGG + Gly-Dap(DNP)-NH2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.70 SrtA
-
 Staphylococcus aureus