EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.12 | phosphatidylserine | - |
Homo sapiens | |
3.1.4.12 | Triton X-100 | - |
Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.4.12 | overexpression of V5-tagged wild-type and mutant nSMases and of GFP-tagged mutant C3A/C5A in MCF-7 cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.4.12 | C1A | site-directed mutagenesis, the altered residue shows altered palmitoylationin the mutant | Homo sapiens |
3.1.4.12 | C2A | site-directed mutagenesis, the altered residue shows altered palmitoylationin the mutant | Homo sapiens |
3.1.4.12 | C3A | site-directed mutagenesis, the altered residue shows altered palmitoylationin the mutant | Homo sapiens |
3.1.4.12 | C3A/C392A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
3.1.4.12 | C3A/C395A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
3.1.4.12 | C3A/C395A/C396A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
3.1.4.12 | C3A/C396A C3A/C400A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
3.1.4.12 | C3A/C5A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
3.1.4.12 | C4A | site-directed mutagenesis, the altered residue shows altered palmitoylationin the mutant | Homo sapiens |
3.1.4.12 | C5A | site-directed mutagenesis, the altered residue shows altered palmitoylationin the mutant | Homo sapiens |
3.1.4.12 | C5A/C53A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
3.1.4.12 | C5A/C53A/C54A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
3.1.4.12 | C5A/C53A/C59A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
3.1.4.12 | C5A/C54A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
3.1.4.12 | C5A/C54A/C59A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
3.1.4.12 | C5A/C59A | site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.4.12 | membrane | catalytic region of nSMase2 localizes to the cytosolic side of the plasma membrane, proposed membrane topology model, overview | Homo sapiens | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.12 | Mg2+ | - |
Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.12 | sphingomyelin + H2O | Homo sapiens | - |
N-acylsphingosine + choline phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.12 | Homo sapiens | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.4.12 | lipoprotein | neutral sphingomyelinase 2 is palmitoylated on multiple cysteine residues, palmitoylation of nSMase2 via thioester linkage, mutational analysis, overview | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.4.12 | MCF-7 cell | mammary carcinoma cell line | Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.12 | sphingomyelin + H2O | - |
Homo sapiens | N-acylsphingosine + choline phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.4.12 | neutral sphingomyelinase 2 | - |
Homo sapiens |
3.1.4.12 | nSMase | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.4.12 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.4.12 | 7.4 | - |
assay at | Homo sapiens |