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Literature summary extracted from

  • Tani, M.; Hannun, Y.A.
    Neutral sphingomyelinase 2 is palmitoylated on multiple cysteine residues. Role of palmitoylation in subcellular localization (2007), J. Biol. Chem., 282, 10047-10056.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.12 phosphatidylserine
-
Homo sapiens
3.1.4.12 Triton X-100
-
Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.12 overexpression of V5-tagged wild-type and mutant nSMases and of GFP-tagged mutant C3A/C5A in MCF-7 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.12 C1A site-directed mutagenesis, the altered residue shows altered palmitoylationin the mutant Homo sapiens
3.1.4.12 C2A site-directed mutagenesis, the altered residue shows altered palmitoylationin the mutant Homo sapiens
3.1.4.12 C3A site-directed mutagenesis, the altered residue shows altered palmitoylationin the mutant Homo sapiens
3.1.4.12 C3A/C392A site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant Homo sapiens
3.1.4.12 C3A/C395A site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant Homo sapiens
3.1.4.12 C3A/C395A/C396A site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant Homo sapiens
3.1.4.12 C3A/C396A C3A/C400A site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant Homo sapiens
3.1.4.12 C3A/C5A site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant Homo sapiens
3.1.4.12 C4A site-directed mutagenesis, the altered residue shows altered palmitoylationin the mutant Homo sapiens
3.1.4.12 C5A site-directed mutagenesis, the altered residue shows altered palmitoylationin the mutant Homo sapiens
3.1.4.12 C5A/C53A site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant Homo sapiens
3.1.4.12 C5A/C53A/C54A site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant Homo sapiens
3.1.4.12 C5A/C53A/C59A site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant Homo sapiens
3.1.4.12 C5A/C54A site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant Homo sapiens
3.1.4.12 C5A/C54A/C59A site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant Homo sapiens
3.1.4.12 C5A/C59A site-directed mutagenesis, the altered residues show altered palmitoylationin the mutant Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.12 membrane catalytic region of nSMase2 localizes to the cytosolic side of the plasma membrane, proposed membrane topology model, overview Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.12 sphingomyelin + H2O Homo sapiens
-
N-acylsphingosine + choline phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.4.12 lipoprotein neutral sphingomyelinase 2 is palmitoylated on multiple cysteine residues, palmitoylation of nSMase2 via thioester linkage, mutational analysis, overview Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.12 MCF-7 cell mammary carcinoma cell line Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 sphingomyelin + H2O
-
Homo sapiens N-acylsphingosine + choline phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 neutral sphingomyelinase 2
-
Homo sapiens
3.1.4.12 nSMase
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.12 37
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.12 7.4
-
assay at Homo sapiens