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Literature summary extracted from

  • Suzuki, H.; Takahashi, S.; Watanabe, R.; Fukushima, Y.; Fujita, N.; Noguchi, A.; Yokoyama, R.; Nishitani, K.; Nishino, T.; Nakayama, T.
    An isoflavone conjugate-hydrolyzing beta-glucosidase from the roots of soybean (Glycine max) seedlings: purification, gene cloning, phylogenetics, and cellular localization (2006), J. Biol. Chem., 281, 30251-30259.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 DNA and amino acid sequence determination and anaylsis, expression analysis by semi-quantitative RT-PCR, stable expression of the 35S-GmICHG:GFP fusion gene in Arabidopsis thaliana T87 cells using an Agrobacterium tumefaciens GV3101(pMP90) transformation system, phylogenetic analysis Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Hg2+ 89% inhibition at 0.1 mM Glycine max
3.2.1.21 additional information no or poor effects by 0.1 mM of Co2+, Cu2+, Mg2+, Mn2+, Ni2+, Zn2+, or EDTA Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.019
-
 daidzein 7'-O-(6''-O-beta-D-malonyl-beta-D-glucoside) pH 7.0, 30°C Glycine max
3.2.1.21 0.025
-
 genistein 7-O-(6''-O-malonyl-beta-D-glucoside) pH 7.0, 30°C Glycine max
3.2.1.21 0.025
-
 daidzein 7'-O-beta-D-glucoside pH 7.0, 30°C Glycine max
3.2.1.21 0.032
-
 genistein 7-O-beta-D-glucoside pH 7.0, 30°C Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 cell wall especially of root hairs Glycine max 5618
-
3.2.1.21 intercellular space of seedling roots Glycine max
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 58000
-
 2 * 58000, SDS-PAGE Glycine max
3.2.1.21 100000
-
 gel filtration Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 additional information Glycine max the enzyme is important in flavonoid-mediated interactions of legumes with microbes ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Glycine max Q08IT7
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.21 glycoprotein
-
 Glycine max

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 native enzyme from roots, 4199fold by ammonium sulfate fractionation, anion and cation exchange chromatography, hydrophobic interaction chromatography, gel filtration, and heparin affinity chromatography, to homogeneity Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 additional information no activity in hypocotyl and cotyledons Glycine max
-
3.2.1.21 root
-
 Glycine max
-
3.2.1.21 root hair
-
 Glycine max
-
3.2.1.21 seedling
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 660
-
 purified native enzyme Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 daidzein 7'-O-(6''-O-beta-D-malonyl-beta-D-glucoside) + H2O
-
 Glycine max daidzein + 6-O-malonyl-beta-D-glucopyranose
-
 ?
3.2.1.21 daidzein 7-O-(6-O-malonyl-beta-D-glucoside) + H2O
-
 Glycine max daidzein + 6-O-malonyl-beta-D-glucopyranose
-
 ?
3.2.1.21 daidzein 7-O-beta-D-glucopyranoside + H2O
-
 Glycine max daidzein + beta-D-glucose
-
 ?
3.2.1.21 genistein 7-O-(6''-O-malonyl-beta-D-glucoside) + H2O preferred substrate Glycine max genistein + 6-O-malonyl-beta-D-glucose
-
 ?
3.2.1.21 genistein 7-O-beta-D-glucoside + H2O low activity Glycine max genistein + beta-D-glucose
-
 ?
3.2.1.21 additional information the enzyme is important in flavonoid-mediated interactions of legumes with microbes Glycine max ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 dimer 2 * 58000, SDS-PAGE Glycine max

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 ICHG
-
 Glycine max
3.2.1.21 isoflavone conjugate-hydrolyzing beta-glucosidase
-
 Glycine max
3.2.1.21 More GmICHGis a member of glycoside hydrolase family 1 Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 30
-
 assay at Glycine max

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 13
-
 genistein 7-O-beta-D-glucoside pH 7.0, 30°C Glycine max
3.2.1.21 22
-
 daidzein 7'-O-beta-D-glucoside pH 7.0, 30°C Glycine max
3.2.1.21 46
-
 daidzein 7'-O-(6''-O-beta-D-malonyl-beta-D-glucoside) pH 7.0, 30°C Glycine max
3.2.1.21 98
-
 genistein 7-O-(6''-O-malonyl-beta-D-glucoside) pH 7.0, 30°C Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 7
-
 assay at Glycine max