Literature summary extracted from

Vandenberghe, I.; Kim, J.K.; Devreese, B.; Hacisalihoglu, A.; Iwabuki, H.; Okajima, T.; Kuroda, S.; Adachi, O.; Jongejan, J.A.; Duine, J.A.; Tanizawa, K.; Van Beeumen, J.
The covalent structure of the small subunit from Pseudomonas putida amine dehydrogenase reveals the presence of three novel types of internal cross-linkages, all involving cysteine in a thioether bond (2001), J. Biol. Chem., 276, 42923-42931.

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.4.9.1	hanging drop vapour diffusion method with 18% (w/v) polyethylene glycol 2000 monomethylether and 50 mM NiCl2	Pseudomonas putida

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.4.9.1	p-Nitrophenylhydrazine	-	Pseudomonas putida

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.4.9.1	8486	-	small subunit, MALDI-TOF mass spectrometry	Pseudomonas putida
1.4.9.1	8597	-	1 * 53917 + 1 * 39234 + 1 * 8597, calculated from amino acid sequence	Pseudomonas putida
1.4.9.1	39234	-	1 * 53917 + 1 * 39234 + 1 * 8597, calculated from amino acid sequence	Pseudomonas putida
1.4.9.1	53917	-	1 * 53917 + 1 * 39234 + 1 * 8597, calculated from amino acid sequence	Pseudomonas putida
1.4.9.1	101700	-	calculated from amino acid sequence	Pseudomonas putida

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.4.9.1	Pseudomonas putida	-	-	-
1.4.9.1	Pseudomonas putida	-	strains ATCC 12633 and IFO 15633	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.4.9.1	-	Pseudomonas putida
1.4.9.1	Superdex 75 gel filtration	Pseudomonas putida

Subunits

EC Number	Subunits	Comment	Organism
1.4.9.1	heterotrimer	1 * 53917 + 1 * 39234 + 1 * 8597, calculated from amino acid sequence	Pseudomonas putida
1.4.9.1	heterotrimer	consists of a four-domain alpha-subunit (about 60000 Da) that harbors a di-heme cytochrome c, a seven-bladed beta-propeller beta-subunit(about 40000 Da) that provides part of the active site, and a small gamma-subunit (about 9000 Da) that contains the cofactor cysteine tryptophylquinone	Pseudomonas putida

Synonyms

EC Number	Synonyms	Comment	Organism
1.4.9.1	QH-AmDH	-	Pseudomonas putida
1.4.9.1	quinohemoprotein amine dehydrogenases	-	Pseudomonas putida

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.4.9.1	cysteine tryptophylquinone	-	Pseudomonas putida
1.4.9.1	heme c	-	Pseudomonas putida
1.4.9.1	heme c	contains two hemes c as redox active groups	Pseudomonas putida

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Release 2023.1 (January 2023)