EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.2.3.31 | 2-mercaptoethanol | 5 mM, required for full activity | Streptomyces sp. | |
4.2.3.31 | Tween-80 | 0.1%, required for full activity | Streptomyces sp. | |
5.5.1.13 | 2-mercaptoethanol | 5 mM, required for full activity | Streptomyces sp. | |
5.5.1.13 | Tween-80 | 0.1%, required for full activity | Streptomyces sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.5.1.13 | geranylgeranyl diphosphate | inhibitory above 0.03 mM | Streptomyces sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.31 | 0.00026 | - |
ent-copalyl diphosphate | 30°C, pH 7.0 | Streptomyces sp. | |
5.5.1.13 | 0.0137 | - |
geranylgeranyl diphosphate | 35°C, pH 5.5 | Streptomyces sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.3.31 | Co2+ | 1 mM, 100% of the activity with Mg2+ | Streptomyces sp. | |
4.2.3.31 | Mg2+ | absolutely required, optimal concentraion 1 mM | Streptomyces sp. | |
4.2.3.31 | additional information | no or very weak activity in presence of Mn2+, Ca2+, Cu2+ and Fe2+ | Streptomyces sp. | |
4.2.3.31 | Ni2+ | 1 mM, 57% of the activity with Mg2+ | Streptomyces sp. | |
4.2.3.31 | Zn2+ | 1 mM, 59% of the activity with Mg2+ | Streptomyces sp. | |
5.5.1.13 | Co2+ | or Zn2+, Mg2+, required. Activation in decreasing order: Mg2+, Zn2+, Co2+ | Streptomyces sp. | |
5.5.1.13 | Mg2+ | or Zn2+, Co2+, required. Activation in decreasing order: Mg2+, Zn2+, Co2+ | Streptomyces sp. | |
5.5.1.13 | Zn2+ | or Mg2+, Co2+, required. Activation in decreasing order: Mg2+, Zn2+, Co2+ | Streptomyces sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.3.31 | 35000 | - |
gel filtration | Streptomyces sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.3.31 | Streptomyces sp. | - |
- |
- |
4.2.3.31 | Streptomyces sp. KO-3988 | - |
- |
- |
5.5.1.13 | Streptomyces sp. | - |
- |
- |
5.5.1.13 | Streptomyces sp. KO-3988 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.31 | ent-copalyl diphosphate | - |
Streptomyces sp. | pimara-9(11),15-diene + diphosphate | - |
? | |
4.2.3.31 | ent-copalyl diphosphate | - |
Streptomyces sp. KO-3988 | pimara-9(11),15-diene + diphosphate | - |
? | |
4.2.3.31 | additional information | no substrates: farnesyl diphosphate, geranylgeranyl diphosphate, terpentecin diphosphate | Streptomyces sp. | ? | - |
? | |
4.2.3.31 | additional information | no substrates: farnesyl diphosphate, geranylgeranyl diphosphate, terpentecin diphosphate | Streptomyces sp. KO-3988 | ? | - |
? | |
5.5.1.13 | geranylgeranyl diphosphate | - |
Streptomyces sp. | ent-copalyl diphosphate | - |
? | |
5.5.1.13 | geranylgeranyl diphosphate | - |
Streptomyces sp. KO-3988 | ent-copalyl diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.3.31 | monomer | x * 35000, SDS-PAGE | Streptomyces sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.3.31 | 30 | - |
0.05 M Tris, pH 7.0 | Streptomyces sp. |
5.5.1.13 | 35 | - |
- |
Streptomyces sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.3.31 | 35 | - |
pH 5.5, stable for at least 1 h | Streptomyces sp. |
5.5.1.13 | 35 | - |
pH 5.5, stable for at least 1 h | Streptomyces sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.31 | 0.0014 | - |
ent-copalyl diphosphate | 30°C, pH 7.0 | Streptomyces sp. | |
5.5.1.13 | 0.033 | - |
geranylgeranyl diphosphate | 35°C, pH 5.5 | Streptomyces sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.3.31 | 7 | - |
- |
Streptomyces sp. |
5.5.1.13 | 5.5 | - |
- |
Streptomyces sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.2.3.31 | 6 | 8 | - |
Streptomyces sp. |
5.5.1.13 | 5 | 8 | more than 90% of maximum activity within this range | Streptomyces sp. |