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Literature summary extracted from

  • de Mendonca, J.D.; Ely, F.; Palma, M.S.; Frazzon, J.; Basso, L.A.; Santos, D.S.
    Functional characterization by genetic complementation of aroB-encoded dehydroquinate synthase from Mycobacterium tuberculosis H37Rv and its heterologous expression and purification (2007), J. Bacteriol., 189, 6246-6252.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.2.3.4 medicine the enzymes of the shikimate pathway are attractive drug targets for the treatment of tuberculosis Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.4 into the pET23a+ vector for expression in Escherichia coli BL21DE3 cells Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.4 Co2+
-
 Mycobacterium tuberculosis
4.2.3.4 Zn2+
-
 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.4 38100
-
 determined by SDS-PAGE Mycobacterium tuberculosis
4.2.3.4 38140
-
 determined by ESI-MS Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.4 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate Mycobacterium tuberculosis
-
 3-dehydroquinate + phosphate
-
 ir
4.2.3.4 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate Mycobacterium tuberculosis H37Rv
-
 3-dehydroquinate + phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.4 Mycobacterium tuberculosis P9WPX9
-
-
4.2.3.4 Mycobacterium tuberculosis H37Rv P9WPX9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.4 using a Q-Sepharose Fast Flow, a HiLoad 16/10 phenyl-Sepharose, and a Sephacryl S-200 HR column Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.4 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate
-
 Mycobacterium tuberculosis 3-dehydroquinate + phosphate
-
 ir
4.2.3.4 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate
-
 Mycobacterium tuberculosis H37Rv 3-dehydroquinate + phosphate
-
 ir

Synonyms

EC Number Synonyms Comment Organism
4.2.3.4 3-dehydroquinate synthase
-
 Mycobacterium tuberculosis
4.2.3.4 dehydroquinate synthase
-
 Mycobacterium tuberculosis
4.2.3.4 DHQ synthase
-
 Mycobacterium tuberculosis
4.2.3.4 DHQS
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.4 25
-
 activity assay Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.4 7.6
-
 activity assay Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.3.4 NADH
-
 Mycobacterium tuberculosis