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Literature summary extracted from

  • Elmi, F.; Lee, H.T.; Huang, J.Y.; Hsieh, Y.C.; Wang, Y.L.; Chen, Y.J.; Shaw, S.Y.; Chen, C.J.
    Stereoselective esterase from Pseudomonas putida IFO12996 reveals alpha/beta hydrolase folds for D-beta-acetylthioisobutyric acid synthesis. (2005), J. Bacteriol., 187, 8470-8476.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 expression in Escherichia coli Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.1 hanging-drop vapor diffusion method, 1.6 A resolution Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 33000
-
 3 * 33000, SDS-PAGE Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Pseudomonas putida Q3HWU8 IFO12996
-
3.1.1.1 Pseudomonas putida IFO12996 Q3HWU8 IFO12996
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 DL-methyl 2-chloropropionate + H2O 25% of the activity with methyl DL-beta-acetylthioisobutyrate Pseudomonas putida ?
-
 ?
3.1.1.1 DL-methyl 2-chloropropionate + H2O as active as with methyl DL-beta-acetylthioisobutyrate Pseudomonas putida ?
-
 ?
3.1.1.1 DL-methyl 2-chloropropionate + H2O 25% of the activity with methyl DL-beta-acetylthioisobutyrate Pseudomonas putida IFO12996 ?
-
 ?
3.1.1.1 DL-methyl 2-chloropropionate + H2O as active as with methyl DL-beta-acetylthioisobutyrate Pseudomonas putida IFO12996 ?
-
 ?
3.1.1.1 ethyl DL-beta-acetylthioisobutyrate + H2O 40% of the activity with methyl DL-beta-acetylthioisobutyrate Pseudomonas putida ?
-
 ?
3.1.1.1 ethyl DL-beta-acetylthioisobutyrate + H2O 40% of the activity with methyl DL-beta-acetylthioisobutyrate Pseudomonas putida IFO12996 ?
-
 ?
3.1.1.1 methyl DL-beta-acetylthioisobutyrate + H2O produces D-beta-acetylthioisobutyrate with a degree of conversion of 49.5% and an enantiomeric excess value of 97.2% at an optimum pH of about 8-10 and an optimum temperature of about 57 to 67°C Pseudomonas putida D-beta-acetylthioisobutyrate + methanol
-
 ?
3.1.1.1 methyl DL-beta-acetylthioisobutyrate + H2O produces D-beta-acetylthioisobutyrate with a degree of conversion of 49.5% and an enantiomeric excess value of 97.2% at an optimum pH of about 8-10 and an optimum temperature of about 57 to 67°C Pseudomonas putida IFO12996 D-beta-acetylthioisobutyrate + methanol
-
 ?
3.1.1.1 additional information no activity with 2-hydroxyethyl DL-beta-acetylthioisobutyrate Pseudomonas putida ?
-
 ?
3.1.1.1 additional information no activity with 2-hydroxyethyl DL-beta-acetylthioisobutyrate Pseudomonas putida IFO12996 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 trimer 3 * 33000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 57 67
-
 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 8 10
-
 Pseudomonas putida