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Literature summary extracted from

  • Bouknight, R.R.; Sadoff, H.L.
    Tryptophan catabolism in Bacillus megaterium (1975), J. Bacteriol., 121, 70-76.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.52 additional information kynurenine induces both tryptophan oxygenase and kynureninase of the catabolic pathway of L-tryptophan degradation Priestia megaterium
3.7.1.3 additional information kynurenine induces both tryptophan oxygenase and kynureninase of the catabolic pathway of L-tryptophan degradation Priestia megaterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.52 hydroxylamine
-
 Priestia megaterium
1.13.11.52 additional information catabolite repression of the pathway by glucose and glutamate, arginine does not cause repression, no inhibition by D-tryptophan Priestia megaterium
1.13.11.52 potassium cyanide
-
 Priestia megaterium
1.13.11.52 Sodium azide
-
 Priestia megaterium
3.7.1.3 additional information catabolite repression of the pathway by glucose and glutamate, arginine does not cause repression Priestia megaterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.52 Fe2+ the enzyme has a functional heme group Priestia megaterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.1 catechol + O2 Priestia megaterium the enzyme is involved in the catabolic pathway of L-tryptophan degradation, overview cis,cis-muconate
-
 ?
1.13.11.52 L-tryptophan + O2 Priestia megaterium the enzyme is involved in the catabolic pathway of L-tryptophan degradation, overview N-formyl-L-kynurenine
-
 ?
3.7.1.3 L-kynurenine + H2O Priestia megaterium the enzyme is involved in the catabolic pathway of L-tryptophan degradation, overview anthranilate + L-alanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Priestia megaterium
-
 strain ATCC 19213
-
1.13.11.52 Priestia megaterium
-
 strain ATCC 19213
-
3.7.1.3 Priestia megaterium
-
 strain ATCC 19213
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.1 culture condition:L-tryptophan-grown cell cells grown in a medium containing L-tryptophan as the sole carbon, nitrogen, and energy source Priestia megaterium
-
1.13.11.52 culture condition:L-tryptophan-grown cell cells grown in a medium containing L-tryptophan as the sole carbon, nitrogen, and energy source Priestia megaterium
-
3.7.1.3 culture condition:L-tryptophan-grown cell cells grown in a medium containing L-tryptophan as the sole carbon, nitrogen, and energy source Priestia megaterium
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.1 0.355
-
-
 Priestia megaterium
1.13.11.52 additional information
-
 poor activity in cells grown on arginine and on glucose, also in presence of L-tryptophan Priestia megaterium
1.13.11.52 12.5
-
 cells grown on L-alanine Priestia megaterium
1.13.11.52 15.6
-
 cells grown on L-asparagine Priestia megaterium
1.13.11.52 22.7
-
 cells grown on L-tryptophan Priestia megaterium
1.13.11.52 23.5
-
 cells grown on L-arginine Priestia megaterium
3.7.1.3 additional information
-
 poor activity in cells grown on arginine and on glucose, also in presence of L-tryptophan Priestia megaterium
3.7.1.3 4.4
-
 cells grown on L-asparagine Priestia megaterium
3.7.1.3 8.8
-
 cells grown on L-arginine Priestia megaterium
3.7.1.3 10.3
-
 cells grown on L-alanine Priestia megaterium
3.7.1.3 31.25
-
 cells grown on L-tryptophan Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 catechol + O2
-
 Priestia megaterium cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2 the enzyme is involved in the catabolic pathway of L-tryptophan degradation, overview Priestia megaterium cis,cis-muconate
-
 ?
1.13.11.52 L-tryptophan + O2 the enzyme is involved in the catabolic pathway of L-tryptophan degradation, overview Priestia megaterium N-formyl-L-kynurenine
-
 ?
1.13.11.52 L-tryptophan + O2 D-tryptophan is no substrate Priestia megaterium N-formyl-L-kynurenine
-
 ?
3.7.1.3 L-kynurenine + H2O
-
 Priestia megaterium anthranilate + L-alanine
-
 ?
3.7.1.3 L-kynurenine + H2O the enzyme is involved in the catabolic pathway of L-tryptophan degradation, overview Priestia megaterium anthranilate + L-alanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.1 catechol oxygenase
-
 Priestia megaterium
1.13.11.52 tryptophan oxygenase
-
 Priestia megaterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.1 25
-
 assay at Priestia megaterium
1.13.11.52 25
-
 assay at Priestia megaterium
3.7.1.3 25
-
 assay at Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 7
-
 assay at Priestia megaterium
1.13.11.52 7
-
 assay at Priestia megaterium
3.7.1.3 7
-
 assay at Priestia megaterium