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Literature summary extracted from

  • Park, K.
    Function and tertiary- and quaternary-structure of cyclodextrin-hydrolyzing enzymes (CDase), a group of multisubstrate specific enzymes belonging to the alpha -amylase family (2006), J. Appl. Glycosci., 53, 35-44.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.54 additional information neopullulanases, cyclomaltodextrinases and maltogenic amylases display similar biochemical properties and share almost the same 3D structures, enzymes should be classified under the same name and enzyme code, cyclodextrins are the most preferred substrates Geobacillus stearothermophilus
3.2.1.54 additional information neopullulanases, cyclomaltodextrinases and maltogenic amylases display similar biochemical properties and share almost the same 3D structures, enzymes should be classified under the same name and enzyme code, cyclodextrins are the most preferred substrates Pyrococcus furiosus
3.2.1.54 additional information neopullulanases, cyclomaltodextrinases and maltogenic amylases display similar biochemical properties and share almost the same 3D structures, enzymes should be classified under the same name and enzyme code, cyclodextrins are the most preferred substrates Bacillus licheniformis
3.2.1.54 additional information neopullulanases, cyclomaltodextrinases and maltogenic amylases display similar biochemical properties and share almost the same 3D structures, enzymes should be classified under the same name and enzyme code, cyclodextrins are the most preferred substrates Thermotoga maritima
3.2.1.54 additional information neopullulanases, cyclomaltodextrinases and maltogenic amylases display similar biochemical properties and share almost the same 3D structures, enzymes should be classified under the same name and enzyme code, cyclodextrins are the most preferred substrates Thermoplasma volcanium
3.2.1.54 additional information neopullulanases, cyclomaltodextrinases and maltogenic amylases display similar biochemical properties and share almost the same 3D structures, enzymes should be classified under the same name and enzyme code, cyclodextrins are the most preferred substrates Nostoc punctiforme
3.2.1.54 additional information neopullulanases, cyclomaltodextrinases and maltogenic amylases display similar biochemical properties and share almost the same 3D structures, enzymes should be classified under the same name and enzyme code, cyclodextrins are the most preferred substrates Bacillus sp. I-5
3.2.1.54 additional information neopullulanases, cyclomaltodextrinases and maltogenic amylases display similar biochemical properties and share almost the same 3D structures, enzymes should be classified under the same name and enzyme code, cyclodextrins are the most preferred substrates Thermus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 Bacillus licheniformis
-
-
-
3.2.1.54 Bacillus sp. I-5
-
-
-
3.2.1.54 Bacillus thermoalkalophilus
-
-
-
3.2.1.54 Geobacillus stearothermophilus
-
-
-
3.2.1.54 Nostoc punctiforme
-
-
-
3.2.1.54 Pyrococcus furiosus
-
-
-
3.2.1.54 Thermoplasma volcanium
-
-
-
3.2.1.54 Thermotoga maritima
-
-
-
3.2.1.54 Thermus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 alpha-cyclodextrin + H2O
-
Geobacillus stearothermophilus maltose + D-glucose
-
?
3.2.1.54 alpha-cyclodextrin + H2O
-
Pyrococcus furiosus maltose + D-glucose
-
?
3.2.1.54 alpha-cyclodextrin + H2O
-
Bacillus licheniformis maltose + D-glucose
-
?
3.2.1.54 alpha-cyclodextrin + H2O
-
Thermotoga maritima maltose + D-glucose
-
?
3.2.1.54 alpha-cyclodextrin + H2O
-
Thermoplasma volcanium maltose + D-glucose
-
?
3.2.1.54 alpha-cyclodextrin + H2O
-
Nostoc punctiforme maltose + D-glucose
-
?
3.2.1.54 alpha-cyclodextrin + H2O
-
Bacillus thermoalkalophilus maltose + D-glucose
-
?
3.2.1.54 alpha-cyclodextrin + H2O
-
Bacillus sp. I-5 maltose + D-glucose
-
?
3.2.1.54 alpha-cyclodextrin + H2O
-
Thermus maltose + D-glucose
-
?
3.2.1.54 beta-cyclodextrin + H2O
-
Geobacillus stearothermophilus maltose + D-glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 beta-cyclodextrin + H2O
-
Pyrococcus furiosus maltose + D-glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 beta-cyclodextrin + H2O
-
Bacillus licheniformis maltose + D-glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 beta-cyclodextrin + H2O
-
Thermotoga maritima maltose + D-glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 beta-cyclodextrin + H2O
-
Thermoplasma volcanium maltose + D-glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 beta-cyclodextrin + H2O
-
Nostoc punctiforme maltose + D-glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 beta-cyclodextrin + H2O
-
Bacillus thermoalkalophilus maltose + D-glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 beta-cyclodextrin + H2O
-
Bacillus sp. I-5 maltose + D-glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 beta-cyclodextrin + H2O
-
Thermus maltose + D-glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 cyclomaltodextrin + H2O
-
Geobacillus stearothermophilus linear maltodextrin
-
?
3.2.1.54 cyclomaltodextrin + H2O
-
Pyrococcus furiosus linear maltodextrin
-
?
3.2.1.54 cyclomaltodextrin + H2O
-
Bacillus licheniformis linear maltodextrin
-
?
3.2.1.54 cyclomaltodextrin + H2O
-
Thermotoga maritima linear maltodextrin
-
?
3.2.1.54 cyclomaltodextrin + H2O
-
Thermoplasma volcanium linear maltodextrin
-
?
3.2.1.54 cyclomaltodextrin + H2O
-
Nostoc punctiforme linear maltodextrin
-
?
3.2.1.54 cyclomaltodextrin + H2O
-
Bacillus thermoalkalophilus linear maltodextrin
-
?
3.2.1.54 cyclomaltodextrin + H2O
-
Bacillus sp. I-5 linear maltodextrin
-
?
3.2.1.54 cyclomaltodextrin + H2O
-
Thermus linear maltodextrin
-
?
3.2.1.54 gamma-cyclodextrin + H2O
-
Geobacillus stearothermophilus maltose + D-glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 gamma-cyclodextrin + H2O
-
Pyrococcus furiosus maltose + D-glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 gamma-cyclodextrin + H2O
-
Bacillus licheniformis maltose + D-glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 gamma-cyclodextrin + H2O
-
Thermotoga maritima maltose + D-glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 gamma-cyclodextrin + H2O
-
Thermoplasma volcanium maltose + D-glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 gamma-cyclodextrin + H2O
-
Nostoc punctiforme maltose + D-glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 gamma-cyclodextrin + H2O
-
Bacillus thermoalkalophilus maltose + D-glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 gamma-cyclodextrin + H2O
-
Bacillus sp. I-5 maltose + D-glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 gamma-cyclodextrin + H2O
-
Thermus maltose + D-glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 maltooligosaccharides + H2O
-
Geobacillus stearothermophilus D-glucose + ?
-
?
3.2.1.54 maltooligosaccharides + H2O
-
Pyrococcus furiosus D-glucose + ?
-
?
3.2.1.54 maltooligosaccharides + H2O
-
Bacillus licheniformis D-glucose + ?
-
?
3.2.1.54 maltooligosaccharides + H2O
-
Thermotoga maritima D-glucose + ?
-
?
3.2.1.54 maltooligosaccharides + H2O
-
Thermoplasma volcanium D-glucose + ?
-
?
3.2.1.54 maltooligosaccharides + H2O
-
Nostoc punctiforme D-glucose + ?
-
?
3.2.1.54 maltooligosaccharides + H2O
-
Bacillus thermoalkalophilus D-glucose + ?
-
?
3.2.1.54 maltooligosaccharides + H2O
-
Bacillus sp. I-5 D-glucose + ?
-
?
3.2.1.54 maltooligosaccharides + H2O
-
Thermus D-glucose + ?
-
?
3.2.1.54 additional information neopullulanases, cyclomaltodextrinases and maltogenic amylases display similar biochemical properties and share almost the same 3D structures, enzymes should be classified under the same name and enzyme code, cyclodextrins are the most preferred substrates Bacillus thermoalkalophilus ?
-
?
3.2.1.54 pullulan + H2O
-
Geobacillus stearothermophilus maltotriose + ?
-
?
3.2.1.54 pullulan + H2O
-
Pyrococcus furiosus maltotriose + ?
-
?
3.2.1.54 pullulan + H2O
-
Bacillus licheniformis maltotriose + ?
-
?
3.2.1.54 pullulan + H2O
-
Thermotoga maritima maltotriose + ?
-
?
3.2.1.54 pullulan + H2O
-
Thermoplasma volcanium maltotriose + ?
-
?
3.2.1.54 pullulan + H2O
-
Nostoc punctiforme maltotriose + ?
-
?
3.2.1.54 pullulan + H2O
-
Bacillus thermoalkalophilus maltotriose + ?
-
?
3.2.1.54 pullulan + H2O
-
Bacillus sp. I-5 maltotriose + ?
-
?
3.2.1.54 pullulan + H2O
-
Thermus maltotriose + ?
-
?
3.2.1.54 starch + H2O
-
Geobacillus stearothermophilus D-glucose + ?
-
?
3.2.1.54 starch + H2O
-
Pyrococcus furiosus D-glucose + ?
-
?
3.2.1.54 starch + H2O
-
Bacillus licheniformis D-glucose + ?
-
?
3.2.1.54 starch + H2O
-
Thermotoga maritima D-glucose + ?
-
?
3.2.1.54 starch + H2O
-
Thermoplasma volcanium D-glucose + ?
-
?
3.2.1.54 starch + H2O
-
Nostoc punctiforme D-glucose + ?
-
?
3.2.1.54 starch + H2O
-
Bacillus thermoalkalophilus D-glucose + ?
-
?
3.2.1.54 starch + H2O
-
Bacillus sp. I-5 D-glucose + ?
-
?
3.2.1.54 starch + H2O
-
Thermus D-glucose + ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.54 dimer
-
Geobacillus stearothermophilus
3.2.1.54 dimer
-
Pyrococcus furiosus
3.2.1.54 dimer
-
Bacillus licheniformis
3.2.1.54 dimer
-
Bacillus thermoalkalophilus
3.2.1.54 dimer
-
Thermus
3.2.1.54 dodecamer
-
Bacillus sp. I-5
3.2.1.54 More high oligomer Thermotoga maritima
3.2.1.54 More high oligomer Thermoplasma volcanium
3.2.1.54 trimer
-
Nostoc punctiforme

Synonyms

EC Number Synonyms Comment Organism
3.2.1.54 CDase
-
Geobacillus stearothermophilus
3.2.1.54 CDase
-
Pyrococcus furiosus
3.2.1.54 CDase
-
Bacillus licheniformis
3.2.1.54 CDase
-
Thermotoga maritima
3.2.1.54 CDase
-
Thermoplasma volcanium
3.2.1.54 CDase
-
Nostoc punctiforme
3.2.1.54 CDase
-
Bacillus thermoalkalophilus
3.2.1.54 CDase
-
Bacillus sp. I-5
3.2.1.54 CDase
-
Thermus
3.2.1.54 cyclomaltodextrinase
-
Geobacillus stearothermophilus
3.2.1.54 cyclomaltodextrinase
-
Pyrococcus furiosus
3.2.1.54 cyclomaltodextrinase
-
Bacillus licheniformis
3.2.1.54 cyclomaltodextrinase
-
Thermotoga maritima
3.2.1.54 cyclomaltodextrinase
-
Thermoplasma volcanium
3.2.1.54 cyclomaltodextrinase
-
Nostoc punctiforme
3.2.1.54 cyclomaltodextrinase
-
Bacillus thermoalkalophilus
3.2.1.54 cyclomaltodextrinase
-
Bacillus sp. I-5
3.2.1.54 cyclomaltodextrinase
-
Thermus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.54 25
-
-
Nostoc punctiforme
3.2.1.54 55
-
-
Geobacillus stearothermophilus
3.2.1.54 60
-
-
Bacillus sp. I-5
3.2.1.54 60
-
-
Thermus
3.2.1.54 70
-
-
Bacillus licheniformis
3.2.1.54 70
-
-
Bacillus thermoalkalophilus
3.2.1.54 75
-
-
Thermoplasma volcanium
3.2.1.54 85
-
-
Thermotoga maritima
3.2.1.54 90
-
-
Pyrococcus furiosus