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Literature summary extracted from
- Immobilization of alpha -glucuronidase specific for O-alpha -D-glucosyluronic acid alpha -D-glucosiduronic acid from Aspergillus niger and production of D-glucuronic acid (2006), J. Appl. Glycosci., 53, 181-185.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.139 | additional information | glutaraldehyde stabilizes | Aspergillus niger |
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.139 | additional information | immobilized enzyme is effective in high yield production of D-glucuronic acid | Aspergillus niger |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.139 | D-glucuronic acid | significantly inhibits in the presence of over 200 mM substrate, complete hydrolysis of 500 mM substrate is accomplished when D-glucuronic acid concentration is kept below 300-400 mM | Aspergillus niger |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.139 | Aspergillus niger | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.139 | partially purified by gel filtration | Aspergillus niger |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.139 | 61 | - |
partially purified enzyme | Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.139 | O-alpha-D-glucosyluronic acid alpha-D-glucosiduronic acid | the immobilized enzyme completely hydrolyzes 100 mM of the substrate | Aspergillus niger | D-glucuronic acid | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.139 | TreDCase | - |
Aspergillus niger |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.139 | 40 | - |
immobilized TreDCase | Aspergillus niger |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.139 | additional information | - |
below 40°C, immobilized TreDCase, treatment with glutaraldehyde does not affect thermostability | Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.139 | additional information | - |
3.0-3.5, immobilized TreDCase | Aspergillus niger |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.139 | 2 | 4 | immobilized TreDCase | Aspergillus niger |
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