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Literature summary extracted from

  • Maresh, J.J.; Giddings, L.A.; Friedrich, A.; Loris, E.A.; Panjikar, S.; Trout, B.L.; Stoeckigt, J.; Peters, B.; OConnor, S.E.
    Strictosidine synthase: mechanism of a Pictet-Spengler catalyzing enzyme (2008), J. Am. Chem. Soc., 130, 710-723.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.3.2
-
 Catharanthus roseus
4.3.3.2 for expression in Escherichia coli Rauvolfia serpentina

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.3.2 cocrystallized with inhibitor 6, the complete data are collected to 3.0 A resolution Rauvolfia serpentina

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.3.2 3-[(tert-butoxycarbonyl)amino]propyl (3R,4S)-3-ethenyl-2-(beta-D-glucopyranosyloxy)-4-(2-oxoethyl)-3,4-dihydro-2H-pyran-5-carboxylate
-
 Catharanthus roseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.3.2 3-alpha(S)-strictosidine + H2O Catharanthus roseus
-
 tryptamine + secologanin
-
 ?
4.3.3.2 3-alpha(S)-strictosidine + H2O Rauvolfia serpentina
-
 tryptamine + secologanin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.2 Catharanthus roseus
-
-
-
4.3.3.2 Rauvolfia serpentina P68175
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.2
-
 Catharanthus roseus
4.3.3.2
-
 Rauvolfia serpentina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.2 3-alpha(S)-strictosidine + H2O
-
 Catharanthus roseus tryptamine + secologanin
-
 ?
4.3.3.2 3-alpha(S)-strictosidine + H2O
-
 Rauvolfia serpentina tryptamine + secologanin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.3.2 strictosidine synthase
-
 Catharanthus roseus
4.3.3.2 strictosidine synthase
-
 Rauvolfia serpentina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.3.2 30
-
 activity assay Catharanthus roseus
4.3.3.2 30
-
 activity assay Rauvolfia serpentina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.3.2 7
-
 activity assay Catharanthus roseus
4.3.3.2 7
-
 activity assay Rauvolfia serpentina

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.3.3.2 0.000003
-
-
 Catharanthus roseus 3-[(tert-butoxycarbonyl)amino]propyl (3R,4S)-3-ethenyl-2-(beta-D-glucopyranosyloxy)-4-(2-oxoethyl)-3,4-dihydro-2H-pyran-5-carboxylate