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Literature summary extracted from

  • Cox, R.S.; Schenk, G.; Mitic, N.; Gahan, L.R.; Hengge, A.C.
    Diesterase activity and substrate binding in purple acid phosphatases (2007), J. Am. Chem. Soc., 129, 9550-9551.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 phosphate
-
 Phaseolus vulgaris
3.1.3.2 phosphate
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 1.25
-
 p-nitrophenyl phosphate at pH 4.9 Sus scrofa
3.1.3.2 2.2
-
 p-nitrophenyl phosphate at pH 6.25 Phaseolus vulgaris
3.1.3.2 110
-
 methyl-p-nitrophenyl phosphate at pH 4.9 Sus scrofa
3.1.3.2 138
-
 methylphosphate at pH 4.9 Sus scrofa
3.1.3.2 281
-
 methylphosphate at pH 6.25 Phaseolus vulgaris
3.1.3.2 356
-
 methyl-p-nitrophenyl phosphate at pH 6.25 Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Phaseolus vulgaris
-
 variety kidney bean
-
3.1.3.2 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 kidney
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 methyl-p-nitrophenyl phosphate + H2O
-
 Phaseolus vulgaris p-nitrophenol + methyl phosphate
-
 ?
3.1.3.2 methyl-p-nitrophenyl phosphate + H2O
-
 Sus scrofa p-nitrophenol + methyl phosphate
-
 ?
3.1.3.2 methylphosphate + H2O
-
 Phaseolus vulgaris methanol + phosphate
-
 ?
3.1.3.2 methylphosphate + H2O
-
 Sus scrofa methanol + phosphate
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Phaseolus vulgaris p-nitrophenol + phosphate
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Sus scrofa p-nitrophenol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 PAP
-
 Phaseolus vulgaris
3.1.3.2 PAP
-
 Sus scrofa
3.1.3.2 purple acid phosphatase
-
 Phaseolus vulgaris
3.1.3.2 purple acid phosphatase
-
 Sus scrofa
3.1.3.2 uteroferrin
-
 Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.2 17
-
 methylphosphate at pH 4.9 Sus scrofa
3.1.3.2 20
-
 methylphosphate at pH 6.25 Phaseolus vulgaris
3.1.3.2 470
-
 p-nitrophenyl phosphate at pH 4.9 Sus scrofa
3.1.3.2 542
-
 methyl-p-nitrophenyl phosphate at pH 4.9 Sus scrofa
3.1.3.2 542
-
 methyl-p-nitrophenyl phosphate at pH 6.25 Phaseolus vulgaris
3.1.3.2 850
-
 p-nitrophenyl phosphate at pH 6.25 Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 4.9
-
-
 Sus scrofa
3.1.3.2 6.25
-
-
 Phaseolus vulgaris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.2 0.8
-
 phosphate using methyl-p-nitrophenyl phosphate as a substrate, at pH 6.25 Phaseolus vulgaris
3.1.3.2 1.8
-
 phosphate using p-nitrophenyl phosphate as a substrate, at pH 6.25 Phaseolus vulgaris
3.1.3.2 4.1
-
 phosphate using methyl-p-nitrophenyl phosphate as a substrate, at pH 4.9 Sus scrofa