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Literature summary extracted from

  • Chen, Y.T.; Hsu, L.H.; Huang, I.P.; Tsai, T.C.; Lee, G.C.; Shaw, J.F.
    Gene cloning and characterization of a novel recombinant antifungal chitinase from papaya (Carica papaya) (2007), J. Agric. Food Chem., 55, 714-722.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 overexpressed in Escherichia coli Carica papaya

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 Ag+ 10 mM, 16.3% loss of activity Carica papaya
3.2.1.14 Cu2+ 10 mM, 60% loss of activity Carica papaya
3.2.1.14 EDTA 10 mM, 6.7% loss of activity Carica papaya
3.2.1.14 Fe3+ 10 mM, 29.3% loss of activity Carica papaya
3.2.1.14 Hg2+ 10 mM, complete loss of activity Carica papaya
3.2.1.14 K+ 10 mM, 7.7% loss of activity Carica papaya
3.2.1.14 Mn2+ 10 mM, 64.7% loss of activity Carica papaya
3.2.1.14 Zn2+ 10 mM, 62.0% loss of activity Carica papaya

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 26000
-
 x * 26000, SDS-PAGE Carica papaya
3.2.1.14 26200
-
 x * 26200, calculated from sequence Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Carica papaya
-
 L. cv. Tainong 2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 fruit
-
 Carica papaya
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.14 30°C, 3 weeks, 36% loss of activity Carica papaya

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 glycol chitin + H2O exochitinase activity that yields p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose, and then when the p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose is accumulated, the p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose is further cleaved into N,N'-diacetylchitobiose and GlcNAc Carica papaya N,N'-diacetylchitobiose + GlcNAc
-
 ?
3.2.1.14 additional information recombinant CpCHI shows antibacterial activity in which 50% of Escherichia coli is inhibited by a 0.0025 mM concentration of the enzyme. Antifungal activity: the spore germination of Alternaria brassicicola can be completely inhibited by a 76 nM level of recombinant CpCHI Carica papaya ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 26000, SDS-PAGE Carica papaya
3.2.1.14 ? x * 26200, calculated from sequence Carica papaya

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 CpCHI
-
 Carica papaya

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 30
-
 purified recombinant enzyme Carica papaya

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 10 70 10°C: 85% of maximal activity, 70°C: about 60% or maximal activity Carica papaya

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 30
-
 3 weeks, 36% loss of activity Carica papaya
3.2.1.14 37
-
 3 weeks, less than 50% loss of activity Carica papaya

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5 9 broad optimal pH-range Carica papaya

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 3 10 pH 4: about 50% of maximal activity, pH 10: about 65% of maximal activity Carica papaya

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 3
-
 3 h, about 70% loss of activity Carica papaya
3.2.1.14 4
-
 3 h, about 30% loss of activity Carica papaya
3.2.1.14 5 9 3 h, no loss of activity Carica papaya
3.2.1.14 10
-
 3 h, about 35% loss of activity Carica papaya

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.14 Carica papaya calculated from sequence
-
 6.32