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Literature summary extracted from

  • Fang, T.Y.; Tseng, W.C.; Pan, C.H.; Chun, Y.T.; Wang, M.Y.
    Protein engineering of Sulfolobus solfataricus maltooligosyltrehalose synthase to alter its selectivity (2007), J. Agric. Food Chem., 55, 5588-5594.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.15 F206W significant decrease in transglycosylation activity Saccharolobus solfataricus
5.4.99.15 F206Y significant decrease in transglycosylation activity Saccharolobus solfataricus
5.4.99.15 F207Y significant decrease in transglycosylation activity Saccharolobus solfataricus
5.4.99.15 F405M catalytic activity comparable to wild-type Saccharolobus solfataricus
5.4.99.15 F405S depending on substrate, 29-97% of wild-type transglycosylation efficiency, hydrolytic activity is 16% of wild-type Saccharolobus solfataricus
5.4.99.15 F405W catalytic activity slightly lower than wild-type Saccharolobus solfataricus
5.4.99.15 F405Y decreased ratio of hydrolysis to transglycosylation Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.15 1.93
-
 maltohexaose mutant F206W, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 2.48
-
 maltohexaose mutant F206Y, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 2.84
-
 maltoheptaose mutant F405S, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 2.92
-
 maltohexaose mutant F207Y, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 3.38
-
 maltoheptaose mutant F405M, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 3.7 5 maltohexaose mutant F405S, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 3.8
-
 maltoheptaose mutant F405Y, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 3.88
-
 maltoheptaose wild-type, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 4.53
-
 maltohexaose wild-type, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 4.63
-
 maltohexaose mutant F405M, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 4.9
-
 maltohexaose mutant F405Y, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 5.71
-
 maltoheptaose mutant F405W, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 5.9
-
 maltohexaose mutant F405W, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 5.94
-
 maltopentaose wild-type, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 6.34
-
 maltopentaose mutant F405Y, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 6.6
-
 maltopentaose mutant F405M, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 7.9
-
 maltopentaose mutant F405W, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 9.25
-
 maltopentaose mutant F405S, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 11.6
-
 maltotetraose mutant F405Y, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 13.2
-
 maltotetraose wild-type, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 19
-
 maltotetraose mutant F405M, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 20.2
-
 maltotetraose mutant F405S, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 20.6
-
 maltotetraose mutant F405W, pH 5.0, 60°C Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.15 Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.15 maltoheptaose
-
 Saccharolobus solfataricus alpha-maltopentaosyl trehalose
-
 ?
5.4.99.15 maltohexaose
-
 Saccharolobus solfataricus alpha-maltotetraosyl trehalose
-
 ?
5.4.99.15 Maltopentaose
-
 Saccharolobus solfataricus alpha-Maltotriosyl trehalose
-
 ?
5.4.99.15 Maltotetraose
-
 Saccharolobus solfataricus ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.99.15 6.11
-
 maltohexaose mutant F206Y, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 11.1
-
 maltohexaose mutant F206W, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 14
-
 maltohexaose mutant F207Y, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 61
-
 maltotetraose mutant F405S, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 138
-
 maltotetraose wild-type, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 139
-
 maltotetraose mutant F405W, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 139
-
 maltotetraose mutant F405Y, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 147
-
 maltoheptaose mutant F405S, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 169
-
 maltohexaose mutant F405S, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 196
-
 maltotetraose mutant F405M, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 197
-
 maltopentaose mutant F405S, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 354
-
 maltopentaose mutant F405Y, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 359
-
 maltohexaose wild-type, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 362
-
 maltohexaose mutant F405Y, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 363
-
 maltoheptaose mutant F405Y, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 364
-
 maltopentaose wild-type, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 381
-
 maltoheptaose mutant F405M, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 383
-
 maltoheptaose wild-type, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 410
-
 maltopentaose mutant F405W, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 414
-
 maltohexaose mutant F405M, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 429
-
 maltoheptaose mutant F405W, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 445
-
 maltohexaose mutant F405W, pH 5.0, 60°C Saccharolobus solfataricus
5.4.99.15 481
-
 maltopentaose mutant F405M, pH 5.0, 60°C Saccharolobus solfataricus